TY  - BOOK
AU  - Jocelyn Q. Gacasan, MD, FPSP
AU  - Basilio, Patricia Anne
AU  - Bautista, Angelo Tadeo
AU  - Dalay, Nerrisa Jayne
AU  - Ermitaño, John Joseph Ericson
AU  - Lee, Chin Chin
AU  - Monjardin, Zairah
AU  - Oab, Babylyn
AU  - Reyes, Diane Eliza
AU  - Rimando, Carlo Angelo
AU  - See, Niczon
AU  - Serra, Mady Ann Grace
ED  - 
TI  - Comparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative
AV  - MT 2018 0009 
PY  - 2018///
CY  - Fairview, Quezon City
PB  - School of Medical Technology, FEU-NRMF
N1  - Includes appendices and bibliographical references; THMT
N2  - Abstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative.   Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative.  The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid.  The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope.  of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone.  Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use.
ER  -