TY  - BOOK
AU  - Damian,Khate Chamia L.
AU  - Eugenio, Catherine Andrea G. 
AU  - Gales, Rowena Ann D. 
AU  - Guzman, Jarrylle Dimple P. 
AU  - Madamba, Arielle Camille S. 
AU  -  Nocino,Chelsea July O.
TI  - Beeswax (Cera alba) as an impregnating and embedding medium of intramuscular tissue in histotechniques: [author]: Khate Chamia L. Damian, Catherine Andrea G. Eugenio, Rowena Ann D. Gales, Jarrylle Dimple P. Guzman, Arielle Camille S. Madamba, Chelsea July O. Nocino
AV  - MT 2024 0032 c.1 
PY  - 2024///
CY  - Quezon City, Philippines
PB  - FEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine
N1  - Includes Appendix; bri'oot
N2  - ABTRACTS:
INTRODUCTION: This study investigated beeswax (Cera alba) as a potential alternative impregnating and embedding medium through assessing its ability to take up stains, and its ability to maintain nuclear and cytoplasmic integrity of intramuscular tissues.
METHOD: This study used experimental design to compare the effectiveness of beeswax and 75 paraffin was in impregnating and embedding intramuscular tissues. Multipla analysis of variance (MANOVA) and pairwise comparison were both utilized to evaluate fine 5 groups: 25% beeswax (with 75% paraffin wax), 50% beeswax (with 50% paraffin wax), 75% beeswax (with 25% paraffin wax), and 100% beeswax and a control group with 100% paraffin wax. The samples underwent standard histological processing, sectioning, and staining and were evaluated by medical technologist and a pathologist using a 5-point Likert scale.
RESULT: Result showed that microscopic examination indicated that the 25% beeswax (with 75 paraffin wax) mixture performed comparably to 100% paraffin wax, maintaining excellent nuclear and cytoplasmic integrity. Higher concentrations of beeswax showed decreased effectiveness, particularly in fine sectioning and stain uptake. The 100% beeswax group exhibited the lowest performance, demonstrating significant challenges in tissue processing quality. Specially, tissue sections embedded in 100% beeswax were prone to fragmentation and showed inconsistent staining results. Additionally, the 50% beeswax (with 50% paraffin wax) and 75% beeswax (25% paraffin wax) groups displayed intermediate performance, with occasionally issues in tissue morphology preservation. Overall, 25% beeswax (with 75 paraffin wax) offered a promising alternative for histological applications

UR  - https://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e3e40223f96ef441b2d220eadd74258
ER  -