TY  - BOOK
AU  - Nuez, Eva Nancy
AU  - Patawaran, Edelwisa
AU  - Paz, Jennelyn
AU  - Perez, Janell
AU  - Quines, Joselito
AU  - Tan, Christine
TI  - THE ANTIMICROBIAL SENSITIVITY EFFECT OF PSIDIUM GUAJAVA  (GUAVA)  LEAVES IN ESCHERICHIA COLI STRAINS ATCC 35218 AND ATCC 25922
AV  - THE M-PHAR ND 0005 
PY  - 0000///ND
N1  - farmakoloji
N2  - The study was conducted at FEU-NRMF Fairview, Quezon City from January 3 to 9, 2000 in order to observe the antimicrobial sensitivity effects and the determination of minimum inhibitory concentration  (MIC)  and minimum bactericidal concentration (MBC) of Psidium guajava (guava) leaves in Escherichia coli strain ATCC 35218 and ATCC 25922.                                        The leaves were gathered air-dried, boiled at 100  C, diluted with distilled water, and sterilized.  From the made decoction,  1 ml of the solution was placed on a series of two-fold test tube dilutions containing 4 ml of distilled water.  An ml of Escherichia coli strain, which was standardized with 0.5 Mc Fahrland Nephelometer standard, were also added to the series of test tubes and was subsequently incubated at 37 C for 24 hours.  A corresponding check plate was also done for well diffusion method of antimicrobial susceptibility test using Mueller Hinton Agar which was incubated at 37 C for 24 hours. After the 24 jhour incubation period, dilutions and check plates were noted for growth, e.g. turbidity and zones of inhibition respectively. MIC was noted in the test tube series. The series of dilutions were correspondingly subcultured using Mc Conkey Agar, incubated for 24 hours at 37 C.  After the said incubation, subcultured plates were noted for growth of opaque. lactose fermenting pink colored colonies.  MBC was noted in the said subcultured plates.                                                                                                                                           Turbidity on the test tubes were observed indicating growth.  Mic was noted at the fourth test tube in the series of test tube dilutions which was confirmed by the check plate through the zone of inhibition noted on the wells of Mueller Hinton Agar.  Upon subsequent subculturing, there were significant growth on all dilutions in the Mc Conkey media.                                                                                                                                                                   In these experiment, we therefore conclude that the Psidium guajava (guava) leaf decoction has an antimicrobial effect,   which is bacteriostatic but not bactericidal, on Escherichia coli strains ATCC 35218 ATcc 25922
ER  -