Alicia A. Aldave, RMT ; Polly Chua-Chan, MD

Commercially Prepared Liquid Dishwashing Soap Versus Phenol in Carbol Fuchsin / Unica May Abregas ; Almira Albarido ; Arnette Marie Arada ; Danielle Belle Briones ; Ma. Teresa Juanir ; Rachel Ann Roslin ; Princess Joy Sadiasa ; Jazel Rose Sevillo ; Danica Solis and Liezl Angealene Vargas. - Fairview, Quezon City School of Medical Technology, FEU-NRMF August 2013 - 23 pages: illustrations, tables, photos: 28 cm.

Includes appendices and bibliographical references.

Abstract: Traditional Ziehl-Neelsen method uses phenol in carbol fuchsin. Liquid phenol is readily absorbed through the skin and has numerous health effects. Among these are skin burns, irreversible eye damage, liver kidney damage and severe irritations of respiratory tract. CNS depression with reproductive and fetal effects upon chronic exposure. Detergents are efficient lipophilic agents and safer to handle compared to phenol. It has milder health effects like transient irritation and has no known chronic effects. This research proposes a substitute for the phenol component of the carbol fuchsin in the traditional Ziehl-Neelsen method of staining acid fast bacilli using commercially prepared liquid dishwashing soap for a safer acid fast staining method. The result of this study showed comparable acid fast staining characteristics using either phenol or commercially prepared liquid soap. The use of commercially prepared liquid soap can therefore be recommended for a safer substitute for phenol in carbol fuchsin.

Thesis - School of Medical Technology

MT 2013 0005