Chocolate Agar Plate Modification with Seed Extract from Cacao Tree (Theobroma Cacao L.) in Culturing Haemophilus Influenzae /
Wilniel Bryan F. Calibuso ; Ryan Kenneth G. Canoy ; Bernadette Jia M. Cruz ; Anne Beatrize U. Cua ; Bonn Joseph H. Dedace ; Ray Arden G. Demotica ; Anjunelle S. Esquivel ; Bryan E. Napuaga ; Chellie Mae D. Serrano and Maria Corina O. Velasco.
- Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017
- 64 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Isolation and identification of certain genus and species of bacteria can be done through growth in a culture medium. One example of such medium is Chocolate Agar Plate (CAP), a non-selective media wherein certain enrichment factors are added to promote or enhance growth of some fastidious bacteria. Haemophilus influenzae are small, non-motile gram negative coccobacilli, and are fastidious by nature. They require factors for growth such as Heme factor and Nicotine Adenine Dinucleotide factor. These requirements are provide in CAP to support its growth. Theobroma cacao is a small evergreen tree that is readily available in the Philippines. In many studies, it was proven that cacao has inhibitory effects to bacteria but not the genus Haemophilus. This study involves the cultivation of a purer culture of H. influenzae through the replacement of the inhibitory agent bacitracin with crude extract of cocoa to see if the modified chocolate agar plate is capable of producing results equal to the standard or greater in terms of suppressing the growth of other contaminant bacteria thus growth of purer culture of H. influenzae. Theobroma cacao seed were ground and extracted via ethanolic extraction the subjected to rotary evaporation that yielded the crude extract and incorporated to the CAP as the experimental plates, Bacitracin CAP served as the control. Three concentrations are prepared from the Theobroma cacao crude extract. 4 organisms were used. Haemophilus influenza, as the organism in focus in the study and other organisms which are the usual undesired organisms. The organisms were inoculated on both the plates and observed for the growth and number of colonies. Results showed that modified CAP with seed extract from cacao tree with the concentration of 3% and 5% have not shown selectivity to H.influenzae while 10% of cacao modified CAP have not shown its selectivity but was able to show how increasing concentration of cocoa extract affect the growth of the selected organism.