Commercially Available Cinnamon (Cinnamomum Zeylanicum Presl) Oil Used as an Alternative Medium to Cedar Wood Oil in Oil Immersion Microscopy /
Coleen Erika G. Afable ; Gianina D. Dacuya ; Jennielyn A. Duran ; Marion Q. Esteves ; Kathleen Mae T. Montes ; Maria Elaiza C. Quiambao ; Didith Diane W. Quirino ; Ver John Dave D. Salvador and John Carlo K. Sanchez.
- Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017
- 42 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Immersion oil contributes to finer resolution and brightness of the image under the microscope.Traditional immersion oil is expensive because it is not locally produced. This study determines if commercially available cinnamon oil, which is cheaper and always available, can be used as a substitute to cedar wood oil as immersion oil. This comparative study utilized experimental research design. This study used ten (10) prepared blood smears, ten (100 gram positive smears and ten (10) gram negative smears for the examination of the visibilty, clarity, effect on stain and on the objective lens using cinnamon oil and cedar wood oil and cedar wood oil. Statistical pacakage for Social Sciences version 20.0 was used in the analysis of data. Data obtained were treated statistically by wilcoxon signed rank test and McNemar test. Based on the gathered data, there is no significant difference in the visibility and clarity of the blood cells and bacterial smears between cinnamon oil and cedar wood oil. With regard to the effect on the stain of the blood smears and bacterial smears, there is also no significant difference between the two oils. It was also found out that the effect that the effect of the cinnamon oil on the objective lens of the microscope is similar to that of cedar wood oil, both can be removed easily and do not dry quickly on the objectives lens of the microscope. Since the p-values in all the tests are higher than the level of significance ( 0.05), the researchers accepted the null hypothesis that there is no significant difference between cinnamon oil and cedar wood oil. Therefore, cinnamon oil can be an alternative medium to cedar wood oil. The results of this study will help the laboratories get equally effective results in oil immersion microscopy at a lower cost.