000			03419nam a2200373 4500
999			_c11049 _d11049
003			PILC
005			20240720153046.0
008			180927b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2018 0009
100			_aJocelyn Q. Gacasan, MD, FPSP _eadviser
111			_eRICO 2018
245			_aComparison of Formalin and Honey with 4% Acetic Acid as Histological Fixative / _cPatricia Anne Basilio ; Angelo Tadeo Bautista ; Nerrisa Jayne Dalay ; John Joseph Ericson Ermitaño ; Chin Chin Lee ; Zairah Monjardin ; Babylyn Oab ; Diane Eliza Reyes ; Carlo Angelo Rimando ; Niczon See and Mady Ann Grace Serra.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF, _c2018
300			_a95 pages: _billustration, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: Formalin has always been the gold standard fixative in histological preservation. Recently, concerns on its detrimental effect to health has been increasing, such as nasopharyngeal cancer. This urge the researchers to continue searching for a safer alternative. Honey has recently been known to be to fix histological tissues. The only problem with honey is its tendency to attract ants, this is where acetic acid's usage is for. Acetic acid, a known fixative and also an ant repellant, hence a combination of honey and acetic acid is believed to be able to compare with formalin as a fixative. The primary objective of this research was to compare the quality of slides fixed in three different fixatives; namely 10% neutral buffered formalin (NBF), 20% honey with 4% acetic acid. The research used an experimental study which formalin is compared to two other fixatives. With the standard procedure of histological techniques, the pork Tissues were divided into three set-ups and fixed in 10% NBF, 20% honey, or 20% acetic acid. Following fixation is dehydration with increasing grades of ethanol, then clearing with xylene, next is impregnation and embedding with paraffin, and then sectioning with microtome. After sectioning, staining of the tissues with routine H&E follows, and finally mounting is done. Then, the slides are ready for viewing under the microscope. of the 90 slides viewed under the microscope, honey and honey with acetic acid have yielded good results in tissue preservation which is slightly less comparable to that of the gold standard, formalin, which has yielded excellent results both grossly and microscopically; with honey and acetic acid showing better results that that honey alone. Therefore, the researchers encourage the use of honey with acetic acid as a safer alternative to formalin. One that is accessible and health-friendly for the future generation to use.
521			_aTHMT
700			_aBasilio, Patricia Anne _eauthor
700			_aBautista, Angelo Tadeo _eauthor
700			_aDalay, Nerrisa Jayne _eauthor
700			_aErmitaño, John Joseph Ericson _eauthor
700			_aLee, Chin Chin _eauthor
700			_aMonjardin, Zairah _eauthor
700			_aOab, Babylyn _eauthor
700			_aReyes, Diane Eliza _eauthor
700			_aRimando, Carlo Angelo _eauthor
700			_aSee, Niczon _eauthor
700			_aSerra, Mady Ann Grace _eauthor
942			_2lcc _cTH