000			03236nam a22003857a 4500
999			_c11052 _d11052
003			PILC
005			20240720153047.0
008			180928b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2018 0006
100			_aRogelda G. Bongat, MD _eadviser
111			_eRico 2018
245			_aThe Potential of Tamarindus Indica Linne. (Fam. Fabaceae) Seed Skin as an Alternative Mordant in Microbiologic Stain / _cLyka V. Asilo ; Erica Camille V. Bondoc ; Angelica A. Chua ; Karmella Dennise L. Creer ; Michaela Louis A. Daluz ; Lloyd Bernard C. Dino ; Aubrey T. Durian ; Ma. Kamille Joyce R. Esguerra ; Maria Christine Joice P. Llobrera ; Joshua Mark M. Panis ; Yvea Faye C. Soberano and Mignon M. Tuazon.
260			_aFairview, Quezon City _bSchool of Medica Technology, FEU-NRMF, _c2018
300			_a26 pages: _billustrations, tables; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: In the field of microbiology, staining has been used as a common auxiliary technique to enhance and distinguish different structures of certain pathogens. A mordant is any chemical that binds with a certain dye or stain and fastens it in its place, such that the dye remains adhered to the organism. This study aimed not only to focus on the potential of Tamarindus indica extract as a substitute mordant in microbiologic staining but also its cost effectiveness and eco-friendly factor compared to that of the commercially prepared mordants. The methods used in this study were followed thoroughly by the researchers in order to obtain the ideal concentration of the substance, as used for the research. In this case, the researchers performed a certain process on the Tamarindus indica seed skin until such time that it became a powdery substance. Moreover, the preparation of an inoculum was carried out, and the isolates were used in the research the cultured as part of the experimental study. Furthermore, a Soxhlet extractor was used to accurately extract the seeds, thus producing a certain amount of tannin needed to obtain its ideal concentration. The researchers used the Gelatin Test to confirm the presence of tannins in the said seed. Finally, a gram stain will be performed as another part of the experimental study along with the use of a commonly used mordant and the one produced in the experimental phase for research, thus producing a potential alternative. A basic research was applied in order to develop such refined innovation.
521			_aTHMT
700			_aAsilo, Lyka V. _eauthor
700			_aBondoc, Erica Camille V. _eauthor
700			_aChua, Angelica A. _eauthor
700			_aCreer, Karmella Dennise L. _eauthor
700			_aDaluz, Michaela Louis A. _eauthor
700			_aDino, LLoyd Bernard C. _eauthor
700			_aDurian, Aubrey T. _eauthor
700			_aEsguerra, Ma. Kamille Joyce R. _eauthor
700			_aLlobrera, Maria Christine Joice P. _eauthor
700			_aPanis, Joshua Mark M. _eauthor
700			_aSoberano, Yvea Faye C. _eauthor
700			_aTuazon, Mignon M. _eauthor
942			_2lcc _cTH