000			03820nam a22003737a 4500
999			_c11055 _d11055
003			PILC
005			20240720153047.0
008			181004b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2018 0003
100			_aMary Denneth R. Fuentes, RMT, MSMT _eadviser
111			_eRico 2018
245			_aAntimicrobial Activity of Squid Ink from Sepioteuthis Lessoniana Férussac (Fam. Loliginidae) Against Methicillin-Resistant Staphylococcus Aureus / _cMariel Jasmine A. Agustine ; Lady Susanna D. Alberto ; Kathlyn Louise C. Durante ; Jordhel D. Ferrer ; Laila Noreen R. Magnaye ; Angelean Chay C. Malilay ; Hazel Grace D. Maranan ; Rachel B. Reyes ; Aileen Mae L. Ricio ; Fleureliza Isabel P. Sotelo and Roseanne F. Villamor.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF, _c2018
300			_a51 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aABSTRACT: The emergence of a complicated Staphylococcus stain, methicillin-resistant Staphylococcus aureus (MRSA) has been shown to cause a variety of serious infections a diseases, mainly skin, blood and surgical site infections. While consequently restricting the choice of antibiotics for treatment, administration has generated worldwide concern over the limited pharmaceutical options; hence the difficulty of treating affected patients. MRSA-associated diseases are attributed to direct skin-to-skin contact, sharing of personal articles, misuse of antibiotics and improper medication. While vancomycin remains the standard drug choice for treatment of diseases caused by MRSA, the researchers wanted to discover if a more naturally-inclined clinical measure will suffice the work just as well as vancomycin, to serve as an alternative antimicrobial in case of vancomycin resistance. The researchers used the bioactive antimicrobial properties to squid ink extract from the species Sepioteuthis lessoniana. This study used an experimental research design by comparative method, comparing the antimicrobial effects of vancomycin to 5 controlled disc of (1) 10μl of squid ink extract with dimethyl sulfoxide, (2) 50μl of squid ink extract with dimethyl sulfoxide, (3) 10μl of dimethyl sulfoxide, (4) 10 μl of hexane on duplicates of Mueller Hinton Agar (MHA) plates inoculated with MRSA. After performing the antimicrobial susceptibility testing (AST) using disc diffusion method and thereafter measuring the zones of inhibition (ZOI: susceptibility must be 15 mm or more) using a Vernier caliper of the 5 different laboratory-acquired MRSA strains after incubation for 18-24 hours at 37°C, the researches discovered that the MRSA strains were resistant to the different crude concentrations of squid ink, obtaining a ZOI of only 6 mm. Thje researchers used Escherichia coli to further study the antimicrobial property of the different crude extractions of squid ink. The same procedure was performed with E. coli. After doing AST and measuring the zones on inhibition, the results showed that the E. coli was still resistant to the squid ink disc.
521			_aTHMT
700			_aAgustin, Mariel Jasmine A. _eauthor
700			_aAlberto, Lady Susanna D. _eauthor
700			_aDurante, Kathlyn Louise C. _eauthor
700			_aFerrer, Jordhel D. _eauthor
700			_aMagnaye, Laila Noreen R. _eauthor
700			_aMalilay, Angelean Chay C. _eauthor
700			_aMaranan, Hazel Grace D. _eauthor
700			_aReyes, Rachel B. _eauthor
700			_aRicio, Aileen Mae L. _eauthor
700			_aSotelo, Fleureliza Isabel P. _eauthor
700			_aVillamor, Roseanne F. _eauthor
942			_2lcc _cTH