000			03700nam a22003377a 4500
999			_c11135 _d11135
003			PILC
005			20240720153054.0
008			181217b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2018 0010
100			_aMark Ryan Domingo, RMT _eadviser
111			_eMICO 2018
245			_aThe Efficacy of Tamarindus Indica Leaf Extract as a Reducing Agent in Thesynthesis of Silver Nanoparticles and Analysis of Antimicrobial Activity / _cJeline Althea G. De Castro ; Jaisatine Acushla P. Hermosado ; Eldred E. Lagajino, Jr. ; Ma. Marinel N. Meracap ; Jedidiah G. Monton ; Mary Jhoanna A. Santos ; Tyra Czarrinnah C. Valerio and Angel Mariel S. Villarama.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF, _c2018
300			_a28 pages: _btables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: The researches aim to test the efficacy of Tamarindusindica leaf extract as a reducing agent in the synthesis of silver nanoparticles. This study aims to know the effectiveness of Tmarindusindica leaf extract as a reducing agent, the antimicrobial activity of silver nanoparticles, and the size and shape exhibited by the silver nanoparticles. The researches started their experiment by preparing the leaf extract at the Chemistry Laboratory of Far Eastern University-Nicanor Reyes Medical Foundation. For the preparation of Tamarindusindica leaf extract, the researches cleaned and dried the collected leaves and then cut into smaller pieces. The leaves were then boiled and the extract from the boiled leaves were then filtered. The researches prepared the silver nanoparticle solution by mixing 90mL of 3M silver nitrate solution with 10mL of the leaf extract to reduce the silver nitrate into silver nanoparticles. A change in color was observed. The researches then characterized the silver nanoparticles using Transmission Electron Microscope (TEM) at the Department of Science and Technology. The presence of silver nanoparticles was observed because it exhibited a size of 7.60998 nm and a spherical shape. The researches then tested the silver nanoparticles for its antimicrobial activity by doing an antimicrobial assay. Each well in two Mueller-Hinton Agar containing gram positive Staphylococcus aureusand gram negative Serratiamarcescenshas two drops of the silver nanoparticle solution in it. tha agars were then incubated for 24 hours at 37°C. A zone of inhibition from both agars were seen. Based on the results of this study, it can be inferred that Tamarindusindica leaf extract is effective as a reducing agent in the synthesis of silver nanoparticles and these AgNPs demonstrated antimicrobial activity against Staphylococcus aureusand Serratiamarcescens. The researches identified certain areas in their research that could be further studied such as the use of other plants ( as well as other plants parts) in the production of an extract, the use of other elements as antimicrobial agents, testing of antimicrobial activity of AgNPs against other bacteria, and the use of other organisms such as viruses and fungi.
521			_aTHMT
700			_aDe Castro, Jeline Althea G. _eauthor
700			_aHermosado, Jaisatine Acushla P. _eauthor
700			_aLagajino, Eldred Jr. E. _eauthor
700			_aMeracap, Ma. Marinel N. _eauthor
700			_aMonton, Jedidiah G. _eauthor
700			_aSantos, Mary Jhoanna A. _eauthor
700			_aValerio, Tyra Czarrinnah C. _eauthor
700			_aVillarama, Angel Mariel S. _eauthor
942			_2lcc _cTH