000			03160nam a22003497a 4500
999			_c11148 _d11148
003			PILC
005			20240720153056.0
008			181217b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2018 0012
100			_aMary Denneth Fuentes, RMT, MSMT _eadviser
111			_eMICO 2018
245			_aThe Effectiveness of Sugar Syrup as a Substitute Fixative for Formalin: _ba Comparative Study / _cMarian Areley K. Aguilar ; Riandolph L. Baloloy ; Andrea May A. Castro ; Athena R. David ; Bryan A. Garingan ; Naomi H. Kato ; Alicia Marie S. Lansang ; Aaron Khryss P. Panag and Anna Kristina A. Reyes.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF, _c2018
300			_a34 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: Formalin is a widely popular fixative used in histopathology of tissues. Due to its ability for fast fixation, economic viability and easy usage it is generally used for effortless processing and array of histological techniques that can execute post-fixation. Routine histological staining methods done in laboratories are based on the application and usage of formalin-fixed, paraffin-embedded tissues. Despite these benefits that can be obtain with formalin, the health and safety risks associated with formalin use is a concern. Screening out regrettable substitutes, a handful of synthetic options like alcoholic fixatives, fixatives for nucleic acids, non-alcoholic substitutes, and fixatives with <10% of formalin are commercially available. Natural fixatives such as different sugars, brown sugars and muscovado sugar specifically, are evaluated by the researches as a potential alternative to formalin usage. Fixation was carried out a room temperature for a period of time of 24 hours. Followed by conventional processing method and the tissue sections will then be examined under light microscope. The researchers may be able to conclude that 25% brown sugar and 25% muscovado sugar is comparable to formalin in terms of tissue fixation. The researchers compared formalin and brown sugar by averaging the results of the pathologist and showed that formalin (18.33) has the same average with 25% brown sugar (18.33), while formalin and muscovado sugar (17.33). The results and observations clearly show that both types of sugar can fix tissue. However, the researchers recommend conducting a further study using a lower concentration of the sugar syrup because it yields better results compared to higher concentrations.
521			_aTHMT
700			_aAguilar, Marian Areley K. _eauthor
700			_aBaloloy, Riandolph L. _eauthor
700			_aCastro, Andrea May A. _eauthor
700			_aDavid, Athena R. _eauthor
700			_aGaringan, Bryan A. _eauthor
700			_aKato, Naomi H. _eauthor
700			_aLansang, Alicia Marie S. _eauthor
700			_aPanag, Aaron Khryss P. _eauthor
700			_aReyes, Anna Kristina A. _eauthor
942			_2lcc _cTH