000			03189nam a22003857a 4500
999			_c11286 _d11286
001			MT 2019 0001
003			PILC
005			20240720153109.0
008			190524b xxu||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _erda
041			_aenglish
050			_aMT 2019 0001
111			_eRICO 2019
245			_aCrude methanol extract of Ipomoea Batatas L. Leaves as primary stain in gram stain technique / _cJocel L. Antonio ; Camellie Grace A. Calisaan ; Caila Mae SD. Capuno ; Dylan Jason V. De Castro ; Angelica F. De Jesus ; Mary Rose S. Dela Cruz ; James Matthew M. Dimaano ; Thea Danille N. Drilon ; Beatriz D. Libunao ; Deanne Nicole Sangalang and Minerva Vrem D. Tominez.
260			_aFairview, Quezon City: _bSchool of Medical Technology, FEU-NRMF, _c2019.
300			_a71 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: Crystal Violet is more commonly known as a dye used in histological staining which is Gram's method of classifying bacteria. Despite of its known usefulness, it also acts as a mitolic poison and a potential carcinogen. While anthocyanin, being a useful colorant in many industries with its possible usefulness in being a colorant in the method of staining, has briught interest in the researchers being sweet potato leaves (Ipomoea batatas L.) as a subject of interest its possibility to be a primary stain in the Gram's stain method. This study determined whether an extract coming from sweet potato leaves is an effective primary stain, the method used in staining the sides is the Gram's stain method. It was done through a crude extract from the Department of Science and Technology-Chemical and Energy Division through methanol extraction, the extract was diluted in concentrations of 25%, 50%, 75%,and 100%. After which, the researchers inoculated isolated bacteria provided by the Far Eastern University - Nicanor Reyes Medical Foundation microbiology laboratory bonto slides which was used for the staining process. After the staining process of Staphylococcus aureus (gram positive ) and Escherichia coli (gram negative) in different concentrations of the extract as a primary stain. it showed that no color was able to adhere with the gram positive organism while no unusual results were given by the gram negative organism. This study concluded that anthocyanin from sweet potato leaves with its purpose as a primary stain was not able adhere to the organisms.
521			_aTHMT
700			_aAntonio, Jocel L. _eauthor
700			_aCalisaan, Camellie Grace A. _eauthor
700			_aCapuno, Caila Mae SD. _eauthor
700			_aDe Castro, Dylan Jason V. _eauthor
700			_aDe Jesus, Angelica F. _eauthor
700			_aDela Cruz, Mary Rose S. _eauthor
700			_aDimaano, James Matthew M. _eauthor
700			_aDrilon, Thea Danille N. _eauthor
700			_aLibunao, Beatriz D. _eauthor
700			_aSangalang, Deanne Nicole _eauthor
700			_aTominez, Minerva Vrem D. _eauthor
942			_2lcc _cTH