000			02922nam a22002537a 4500
001			M BIO 2011 0002
003			PILC
005			20241010133111.0
008			241010b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _erda
041			_aenglish
050			_aM BIO 2011 0002
245			_aConversion of Blood Type A to Blood Type O red cells by Endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum _cAnna Belen Alensuela M.D. & FEU-NRMF Medicine Section D Class of 2010-2011
260			_aFairview, Quezon City, Philippines _bDepartment of Biochemistry, FEU-NRMF _c2011
300			_billustrations, tables , photos _c28cm
336			_ardacontent
337			_aradamedia
338			_ardacarrier
520			_aConsidering the fact that there is a high occurrence of hemolytic reactions due to the imbalance figures between the supply and demand of blood (not only in the Philippines, but also worldwide), it is crucial to find for an alternative that could prevent such unwanted incidences. This study primarily aimed to significantly contnbute on the possibility of reducing the morbidity and mortality rates due to incorrect blood transfusion and shoitage of blood supply by exploring the potential for the conversion of blood type A to O, which is consideied the universal donor. This study focused on the use of Bifidobacterium longum, (obtained from Pine Fiee ^e Dried Bifidobacterium powder, Pro-LSB food supplement tablets, and a commercially available yogurt grown in a Bifidobacterium Selective Media (BSM) agar,) which contains an enzyme endo-alpha-Nacetylgalactosaminidase that may specifically convert blood type A red cells to blood type O. Each sample from the three different sources was mixed with 5 drops of blood type A and tested for their effect. Each prepared blood sample with the Bifidobacterium longum was incubated and checked for their agglutination at an interval of 5 minutes, JOminutes, 60 minutes, 1,440 minutes (24 hours) and 2,880 minutes (48 hours). The resulting blood type was determined through the use of an anti-sera and the absence of agglutination after the reaction of the enzyme is indicative for blood Type O. The results showed that the longer the bacteria and blood are exposed together, the agglutination decreases greatly from a strong agglutinate to a weaker agglutinate. Though it has been observed that this idea can be conceivably developed further, it is suggested by the researchers that more tests, experiments, variables and factors be considered to fully determine its mechanism in converting a specific blood typ
521			_abioquimica
700			_aAnna Belen Alensuela, M.D. & FEU-NRMF Medicine Section D Class of 2010-2011
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=7008a3a08a0d7b4ffcf4019bec972a00 _yClick here for FULL TEXT _1ALL
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