000			03558nam a22003737a 4500
001			T002881
003			PILC
005			20241028103154.0
008			241028b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0025 c.1
100	1		_aAcosta, Marier Divine L., _eauthor
245	0	1	_a: A comparative study between glacial acetic acid and citrofortunella aurantifolia (key lime) extract as white blood cell diluting fluid in leukemic blood samples _b[author]Acosta, Marier Divine L., Arias, Shaira Angela B., Bringas, Manuel Alexander II B., Esguerra, John Leuvin C. Grande, Allyssa Marie B., Licup, Roni Margaret N., Macapallag, Maria Amor, Maningas, Andrea Beatrice A., Mendoza, Ma. Andrea Dominique L., Nava, Zoe Maree D., Perez, John Renmar C.
260			_aQuezon City, Philippines, _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation _c2024
300			_a112 pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABSTRACT: Accurate white blood cell (WBC) counting lies in establishing a definitive WBC diluting fluid. The study undertakes a comparative analysis between glacial acetic acid, an established WBC diluting fluid, and Citrofortunella aurantifolia (key lime) extract in terms of their ability to lyse red blood cells (RBCs), preserve WBC visibility, and facilitate WBC counting. The participants' blood samples were divided into two: a control group (healthy individuals), and an experimental group (a patient diagnosed with leukemia). The key lime extract was subjected to a dilution process with normal saline solution (NSS) in three different ratios - 1:2, 1:3, and 1:4. The key lime and NSS solution were centrifuged at 5,000 rpm for 5 minutes, collecting the supernatant as the diluting fluid. The blood samples collected from participants were treated with C. aurantifolia diluting fluid and commercially prepared 2% glacial acetic acid. The samples were examined by two registered medical technologists using a counting chamber and graded according to their efficiency of RBC lysis, WBC visibility, and WBC count to assess both diluting fluids. A1:2 dilution of key lime extract (lmL key lime extract: 2mL NSS) demonstrated nearly the same effect as glacial acetic acid. The researchers concluded that key lime extract was comparable to glacial acetic acid in preserving WBC visibility and facilitating WBC count. However, it was not as efficient as commercially prepared glacial acetic acid, particularly in the lysis of RBCs. Further, the efficacy is limited only for samples with blood counts within normal range, and not for leukemic samples due to its high WBC count. Keywords: Blood Cell Analysis, Hematology, Red Blood Cell Lysis, White Blood Cell Counting
521			_abri'oot
700			_aArias, Shaira Angela B., _eauthor
700			_aBringas, Manuel Alexander II B., _eauthos
700			_aEsguerra, John Leuvin C. _eauthor
700			_aGrande, Allyssa Marie B _eauthor
700			_aLicup, Roni Margaret N., _eauthor
700			_aMacapallag, Maria Amor, _eauthor
700			_aManingas, Andrea Beatrice A., _eauthor
700			_aMendoza, Ma. Andrea Dominique L., _eauthor
700			_aNava, Zoe Maree D., _eauthor
700			_aPerez, John Renmar C. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=7757a64b614ac976f71ca225e745c53c _yClick here for FULL TEXT _1ALL
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