000			03103nam a22003137a 4500
001			T002904
003			PILC
005			20241029150108.0
008			241029b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0032 c.1
100	1		_aDamian,Khate Chamia L. _eauthor
245	0	1	_aBeeswax (Cera alba) as an impregnating and embedding medium of intramuscular tissue in histotechniques _b[author]: Khate Chamia L. Damian, Catherine Andrea G. Eugenio, Rowena Ann D. Gales, Jarrylle Dimple P. Guzman, Arielle Camille S. Madamba, Chelsea July O. Nocino
260			_aQuezon City, Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a84pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABTRACTS: INTRODUCTION: This study investigated beeswax (Cera alba) as a potential alternative impregnating and embedding medium through assessing its ability to take up stains, and its ability to maintain nuclear and cytoplasmic integrity of intramuscular tissues. METHOD: This study used experimental design to compare the effectiveness of beeswax and 75 paraffin was in impregnating and embedding intramuscular tissues. Multipla analysis of variance (MANOVA) and pairwise comparison were both utilized to evaluate fine 5 groups: 25% beeswax (with 75% paraffin wax), 50% beeswax (with 50% paraffin wax), 75% beeswax (with 25% paraffin wax), and 100% beeswax and a control group with 100% paraffin wax. The samples underwent standard histological processing, sectioning, and staining and were evaluated by medical technologist and a pathologist using a 5-point Likert scale. RESULT: Result showed that microscopic examination indicated that the 25% beeswax (with 75 paraffin wax) mixture performed comparably to 100% paraffin wax, maintaining excellent nuclear and cytoplasmic integrity. Higher concentrations of beeswax showed decreased effectiveness, particularly in fine sectioning and stain uptake. The 100% beeswax group exhibited the lowest performance, demonstrating significant challenges in tissue processing quality. Specially, tissue sections embedded in 100% beeswax were prone to fragmentation and showed inconsistent staining results. Additionally, the 50% beeswax (with 50% paraffin wax) and 75% beeswax (25% paraffin wax) groups displayed intermediate performance, with occasionally issues in tissue morphology preservation. Overall, 25% beeswax (with 75 paraffin wax) offered a promising alternative for histological applications.
521			_abri'oot
700			_aEugenio, Catherine Andrea G. _eauthor
700			_aGales, Rowena Ann D. _eauthor
700			_aGuzman, Jarrylle Dimple P. _eauthor
700			_aMadamba, Arielle Camille S. _eauthor
700			_a Nocino,Chelsea July O. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e3e40223f96ef441b2d220eadd74258 _yClick here for FULL TEXT _1ALL
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999			_c13162 _d13162