000			03139nam a22003017a 4500
001			T002907
003			PILC
005			20241030130233.0
008			241030b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0033 c.1
100	1		_aComaling, Carl Allen M. _eauthor
245	0	1	_aCitrus maxima (Pomelo) D- limonene volatile oil extract as a deparaffinization agent in histopathology _b[author]: Carl Allen M. Comaling, Chelsee Yvhon G. Julo, Cris May Bernadette A. Limbaga, Lyca R. Lumaad, Gwyneth Ann P. Masucol, Mark Alexander V. Umipig
260			_aQuezon City, Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a76pages _c28cm
336			_2rdacontext _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABTRACTS: INTRODUCTION: Xylene is commonly used in laboratories, primarily in histopathology, in order to enhance the transparency of tissue slides. However, it posed significant risks, including flammability and adverse health effects from inhalation or skin exposure. Consequently, the research investigated alternative substances, such as D-limonene METHOD: Data were collected from observation made by pathologists, employing a methodology that involved manipulating a single variable to examine its effect on another over a nine-month period. The data were organized into a table, and the Mann-Whitney U Test was used to assess the significance and efficacy of the deparaffinization agents. This test was carried out with IBM SPSS, and the p-value was set at 0.05. A pathologist evaluated the parameters using a scoring criterion. The Citrus maxima volatile extract was first obtained using steam distillation and then concentrated through evaporation. RESULT: The study revealed that the volatile oil, which includes D-limonene, efficiently deparaffinized tissue specimens while Keeping the integrity. The Treated slides were assessed as good to excellent, with a few variations supporting their diagnostic relevance. Based on its efficacy in deparaffinization, Staining quality, and cell morphology preservation, Citrus maxima (Pomelo) D-limonene volatile oil extract appears to be a feasible and perhaps less hazardous clearing agent compared to xylene for tissue processing histopathology. CONCLUSION: The study emphasized the necessity of investigating less hazardous deparaffinization methods, providing useful insights for medical technology, education, and lowering the health-associated risk with standard reagents such as xylene. KEYWORDS: Xylene, Mann-Whitney U Test, IBM SPSS, volatile, Steam Distillation evaporation
521			_abri'oot
700			_aJulo, Chelsee Yvhon G. _eauthor
700			_aLimbaga, Cris May Bernadette A. _eauthor
700			_a Masucol, Gwyneth Ann P. _eauthor
700			_aUmipig Mark Alexander V. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=b657e41b3148434776fee4f01b775ed1 _yClick here for FULL TEXT _1ALL
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