000			03142nam a22003257a 4500
001			T002898
003			PILC
005			20241030140222.0
008			241030b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0031 c.1
100	1		_aCabansag, Almarie F _eauthor
245	0	1	_aAn investigation on the gelling properties of opuntia ficus indica (prickly pear cactus) in correlation to making an alternative culture medium _b[author]: Cabansag, Almarie F.,Cipres, Christelle Anne Joy L., Curioso, Alexandrine Laurice R., Guzman, Alexandra M. Linguete, Thania A., Sangao, Dan Enrique G.,Zonio, Kyte J.
260			_aQuezon City, Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a49pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABSTRACT BACKGROUND: The microbiology department in medical laboratories requires effective methods to cultivate diverse bacterial species. This study explored the potential of Opuntia ficus indica (Prickly Pear Cactus) sap as a plant-based culture medium, which could be more accessible and environmentally friendly. METHOD: This study utilized Three experimental plated mediums: Plate 1, comprising 1000 Ml distilled water and 25g of plant saps; Plate 2, consisting of 1000 mL distilled water and 30g of plant saps; and Plate 3, a modified solution of 970 mL distilled water, 25g of plant saps, and an additional 6g of agar. The physical properties of these media—color, transparency, and consistency—were observed. The consistency was rated on a scale where 1 indicated a watery consistency, and 4-5 indicated solidification. An independent T-test compared the plant-based media with traditional Nutrient agar, using a significance threshold of p < 0.05. RESULTS: Both Plate 1 and Plate 2, with increasing concentrations of cactus sap, resulted in a liquid medium. Plate 3, which included agar, produced a viscous medium. None of the media supported bacterial colony formation due to their liquid or viscous states. CONCLUSION: The results of these experiments indicate that the cactus sap remained liquid across trials without agarose, indicating it lacked necessary gelling agents. While Opuntia ficus indica sap showed potential as a component in culture media, it cannot independently function as a culture medium for bacterial growth without modification or the addition of external gelling agents. Keywords: cactus sap, gelation properties, nutrient agar, plant-based culture media
521			_abri'oot
700			_aCipres, Christelle Anne Joy L _eauthor
700			_aCurioso, Alexandrine Laurice R., _eauthor
700			_aGuzman, Alexandra M. _eauthor
700			_aLinguete, Thania A., _eauthor
700			_aSangao, Dan Enrique G _eauthor
700			_a.,Zonio, Kyte J. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=a19638add20d33a45a6dc126f702e3fa _yClick here for FULL TEXT _1ALL
942			_2lcc _cTH
999			_c13169 _d13169