000			03548nam a22003137a 4500
001			T002895
003			PILC
005			20241030142720.0
008			241030b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0032 c.1
100	1		_aAzurin, Krizzha Faye P. _eauthor
245	0	1	_aExploring the viability of tara gum ( caesalpinia spinosa) as a gelling agent for culture media, _b[author]: Azurin, Krizzha Faye P.,Dela Cruz, Allyson Dominic F., Gaspar, Maybelle Kate C., Lequin, Aarin Nicole N., Lucas, Jessica Marie I., Monteron, Emil John R.
260			_aQuezon City,Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a71pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABSTRACT BACKGROUND: This study aimed to formulate culture media using a different kind of agar, Tara Gum (Caesalpinia spinosa) used as a gelling agent in making culture media. Tara Gum has potential nutrients that would help to promote growth of microorganisms making a cost efficient and sustainable culture media that can be used in microbiology. The study aims to promote the growth of Escherichia coli and Staphylococcus aureus using the proposed agar, Tara Gum. METHOD: The commercially prepared Tara Gum used in culture media making, it will be the gelling agent to be used. Using a 1:1 ratio 12g of broth and agar dissolved into 250mL of distilled water, it will undergo culture media making with the use of laboratory instruments such as analytical balance to measure exact quantity of broth and agar, magnetic stirrer and hot plate to heat the mixture and dissolve completely, and autoclave for sterilization. Making a culture media that may be used in promoting the growth of Escherichia coli and Staphylococcus aureus. RESULT: The result of formulating to develop culture media using Tara Gum was not successful in having the gellike structure that every culture media should be. It results in viscous consistency with 1:1 ratio of broth and Tara Gum. Having different measurements by testing 0.5%, 1%, 1.5% and 2% concentrations to get the proper formula to create gel-like consistency, it remains a liquid to viscous state that cannot be used to perform in inoculating microorganism. This Led to the unsuccessful secondary goal of the study to promote the growth of Escherichia coli and Staphylococcus aureus using the proposed gelling agent Tara Gum. CONCLUSION: Based on the result, the Tara Gum needed more in-depth study as gelling agent to produce a gellike culture media suitable for bacterial growth. Having inadequate data of Tara Gum, it may require additional thickening agent to stabilize culture in the cooling stage. The study advice to perform such comparative study to determine the full potential of Tara Gum with different gelling agents to understand the advantages and limitations. KEYWORDS: Agar, Gel-like Consistency, Tara Gum, Thickening Agent, Stabilize, Tara gum
521			_abri'oot
700			_aDela Cruz, Allyson Dominic F., _eauthor
700			_aGaspar, Maybelle Kate C., _eauthor
700			_aLequin, Aarin Nicole N., _eauthor
700			_aJessica Marie I., _eauthor
700			_aMonteron, Emil John R. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=d44d117e9c5a238e1d7ff163a6248afa _yClick here for FULL TEXT _1ALL
942			_2lcc _cTH
999			_c13170 _d13170