000			03313nam a22003137a 4500
001			T002892
003			PILC
005			20241030144927.0
008			241030b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0028 C.1
100	1		_aArias, Leah Jean P., _eauthor
245	0	1	_aEfficacy of palm wax as an impregnation and embedding medium for tissue processing _b[author]: Arias, Leah Jean P., Bosi, Ma. Zenaida Rose Dc., Briones, Allyana Marie C., Pingol, Doreena Raissa D., Redilosa, Edd Angeline Reigne I., Sangcap, Sarah Mae B.
260			_aQuezon City, Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a86pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABSTRACT Background: Palm wax, a natural and eco-friendly alternative, is being explored as a tissue impregnation and embedding medium due to its potential benefits over traditional paraffin wax. Method: The assessment involved three medical technologists and one pathologist who analyzed palm wax's effectiveness through several criteria: solidification, impregnation, embedding, sectioning, ease of fishing out, stain uptake, and tissue morphology. The evaluation was conducted using pork tissue samples, testing and observing the capability of palm wax in tissue processing using different concentrations. The experimental setup included three tissue blocks and slides for paraffin wax as control and twelve blocks and slides for palm wax with varying concentrations. Results: The results were analyzed using the chi-square goodness of fit test and presented in a semi-tabular format for clarity. Results indicated that palm wax and paraffin wax performed similarly in most aspects. Both waxes melted at comparable temperatures at 60°C and provided clear tissue embedding. The use of Palm wax showed minimal issues with embedding, however, blocks (C and D) have shown some cracks compared to blocks (A and B), which produced tissue ribbons. Extraction ease varied, with Block A being easily removed in a warm water bath, while Block B required a cold-water bath. Stain uptake was comparable between the two media, but pathologist observations revealed vacuolations in Block B, suggesting incomplete impregnation. Tissue morphology varied, with some sections showing no distortion while others experienced cell structure issues, possibly due to the extraction process. Conclusion: Overall, palm wax demonstrated effectiveness as an impregnation and embedding medium in terms of statistical analysis. Further optimization is needed to address issues related to impregnation and tissue morphology. Keywords: Palm Wax, Paraffin Wax, Impregnation and Embedding
521			_abri'oot
700			_aBosi, Ma. Zenaida Rose Dc., _qauthor
700			_aBriones, Allyana Marie C., _eauthor
700			_aPingol, Doreena Raissa D., _eauthor
700			_aRedilosa, Edd Angeline Reigne I., _eauthor
700			_aSangcap, Sarah Mae B. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=8a1bd7229e93035ef640504578a9b4ea _yClick here for FULL TEXT _1ALL
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