000			03378nam a22003257a 4500
001			T002901
003			PILC
005			20241031085900.0
008			241031b |||||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _drda
050			_aMT 2024 0034 c.1
100	1		_aFirmeza, Verlie Jean S., _eauthor
245	0	1	_aSweet orange (citrus sinensis) syrup as a potential routine fixative _b[author]: Firmeza, Verlie Jean S., Guanzon, Jojie Camille G., Lansangan, Jhullian Antonio B., Mendoza, Bianca Katrina G. Musico, Van Rowin F,, Rosales, Angela Clarke R., Tugado, Ma. Cristina J.
260			_aQuezon City,Philippines; _bFEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine, _c2024
300			_a65 pages _c28cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes Appendix
520			_aABSTRACT Introduction The fixation of tissues is critical in histopathology, achieved using formalin, a toxic and carcinogenic substance. The necessity for safer and environmentally friendly alternatives has led to the exploration of natural fixatives. Sweet orange (Citrus sinensis) syrup, with its natural antibacterial, antioxidant, and tissue-preserving properties, presents a potential substitute. This study aims to evaluate the effectiveness of sweet orange syrup as fixative compared to formalin. Primary objectives include assessing the syrup's ability to prevent autolysis and putrefaction, maintain staining quality, and preserve tissue morphology. Methodology Experimental design was employed, using pork liver tissues divided into four groups: standard 10% formalin, 60% sweet orange syrup, 80% sweet orange syrup, and distilled water. The tissues underwent fixation, processing, staining, and microscopic examination. Inferential statistics were used to compare the effectiveness of the fixatives. Results: 60% sweet orange syrup showed comparable staining quality and nuclear detail preservation to formalin but failed to maintain cytoplasmic details and tissue hardening. The 80% syrup and distilled water were less effective overall. The sweet orange syrup's antibacterial properties were insufficient to prevent putrefaction as efficiently as formalin. While the 60% sweet orange syrup demonstrated potential as a natural fixative (staining and nuclear detail preservation), its inconsistent performance in cytoplasmic detail preservation and tissue hardening limits its efficacy as a comprehensive fixative. Recommendation Further research is recommended to enhance the efficacy of sweet orange syrup, such as optimizing concentration and extraction methods to increase phenolic yield. Investigating fixatives' combination might provide more effective alternatives. Keywords: Citrus sinensis, Fixative, Formalin, Histopathology, Tissue processing
521			_abri'oot
700			_aGuanzon, Jojie Camille G., _eauthor
700			_aLansangan, Jhullian Antonio B., _eauthor
700			_aMendoza, Bianca Katrina G. _eauthor
700			_aMusico, Van Rowin F,, _eauthor
700			_aRosales, Angela Clarke R., _eauthor
700			_aTugado, Ma. Cristina J. _eauthor
856			_21 _30 _qpdf _uhttps://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=f2367a4cb623f632964fa143377f6c72 _yClick here for FULL TEXT _1ALL
942			_2lcc _cTH
999			_c13173 _d13173