000			03071nam a22003617a 4500
999			_c8596 _d8596
003			PILC
005			20240720152646.0
008			160505b2008 xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2008 0001
100			_aAlicia Aldave, RMT, MNPEd ; Polly Chua-Chan, MD _eadviser
111			_eGroup 3 | MICO 2008
245			_aAntimicrobial Effect of Calamansi Pulp Extract on Respiratory Bacterial Pathogens / _cAubrey Castro ; Vito Daza ; Liahona De Leon ; Philip Edep ; Ma. Pia Fetizanan ; Rachelle Magpoc ; Karisse Santos ; Paolo Samson ; Eric Tapang and Joseph Tolentino.
260			_aFairview, Quezon City _bFEU-NRMF _c2008
300			_a35 page: _billustrations, tables, photos: _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: The study is entitled as "antimicrobial effect of calamansi pulp extract to respiratory bacterial pathogens." The researcher utilized calamansi pulps extract and tested it against Streptococcus pneumoniae, Streptococcus pyogenes and Klebsiella pneumoniae. This was to dertermine the Minimum Inhibitory Concentrate (MIC) & Minimum Bactericidal Concentrate (MBC) of Calamansi Pulp Extract on the three said organism. Distilled water used to make calamansi pulp extract. This was then subjected to a serial two fold dilution using sterile distilled water from tube 1 to 10. A loop full of bacterial suspension was inoculated to each tube except the negative control. Positive and negative controls were also utilized to compare the results. After which, the suspension is made to stand at room temperature for 30 minutes to allow microbial contact with the extract. At the end of 30 minutes, a loop full from each tube is subcultured on trypticase soy broth to determine the bacteriostatic effect of calamansi extract against Streptococcus pneumoniae, Streptococcus pyogenes and Klebsiella pneumoniae. This would also reveal the Minimum Inhibitory Concentration (MIC). After that, those tubes with no growth as indicated by no visual turbidity or clear would be subcultured on BAP or MacConkey. This is to determine the bacterial effect of calamansi extract against Streptococcus pneumoniae, Streptcoccus pyogenes and Klebsiella pneumoniae. This result would also suggest the Minimum Bactericidal Concentration (MBC). At the end of the study, it was proven that the Calamansi Pulp Extract exhibited bacteriostatic and bactericidal effect against Streptococcus pneumonia, Strepyococcus pyogenes and Klebsiella pneumonia.
521			_aTHMT
700			_aCastro, Aubrey _eauthor
700			_aDaza, Vito _eauthor
700			_aDe Leon, Liahona _eauthor
700			_aEdep, Philip _eauthor
700			_aFetizanan, Ma. Pia _eauthor
700			_aMagpoc, Rachelle _eauthor
700			_aSantos, Karisse _eauthor
700			_aSamson, Paolo _eauthor
700			_aTapang, Eric _eauthor
700			_aTolentino, Joseph _eauthor
942			_2lcc _cTH