000			02283nam a22002897a 4500
999			_c8660 _d8660
001			M BIO 2012 0006
003			PILC
005			20240720152651.0
008			160505b2012 xxu||||| |||| 00| 0 eng d
040			_beng _cFEU-NRMF MEDICAL LIBRARY _erda
041			_aenglish
050			_aM BIO 2012 0006
245			_aIsolation of the hypouricemic components of methanolic leaf extract of persea americana mill in hyper-induced ICR mice / _cSection C1; Joel L. Javate; Mari-Ann Bringas; Dolores Viliran.
260			_aFairview, Quezon City: _bDepartment of Biochemistry, FEU-NRMF, _c2012.
300			_a86 pages: _billustration, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: This study investigated the hypouricemic effect of Persea Americana Mill (avocado) leaf extract (PAE) fraction on serum uric acid levels of ICR mice. Crude PAE was partitioned into hexane and ethyl acetate extracts and then subjected to vacuum column (VLC) chromatography to isolate the components present. Thin layer chromatography (TLC) was employed to check for fraction purity. The ICR mice were induced using potassium oxonate into a hyperuricemic state. Subsequently, the PAE fractions and controls were administered and blood was tested for uric acid levels. Nearly all of the PAE fractions had a hypouricemic effect: namely, one ethyl acetate fraction (70%H: 30%E) and two hexane fractions (60%H: 40%E and 20%H: 80%E) exhibited an even greater reduction in serum uric acid levels than Allopurinol, the gold standard pharmaceutical used for treatment of hyperuricemia. The fraction components were identified as flavonoid derivatives using infrared (IR) spectroscopy. However, a more specific determination of the flavonoids fell outside of the flavonoids fell outside of the limitations of this study. The data made available here obliges further into avocado extract as an alternative treatment for hyperuricemia
521			_aTHDBN
700			_aSection C1 _eauthor
700			_aJavate, Joel L., MD. _eadviser
700			_aBringas, Mari-Ann, MD. _eadviser
700			_aViliran, Dolores, MD. _eadviser
942			_2lcc _cRU