000			03564nam a22003617a 4500
999			_c9953 _d9953
003			PILC
005			20240720152909.0
008			170726b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2017 0006
100			_aAlicia A. Aldave _eadviser
111			_dJune 2017
245			_aChocolate Agar Plate Modification with Seed Extract from Cacao Tree (Theobroma Cacao L.) in Culturing Haemophilus Influenzae / _cWilniel Bryan F. Calibuso ; Ryan Kenneth G. Canoy ; Bernadette Jia M. Cruz ; Anne Beatrize U. Cua ; Bonn Joseph H. Dedace ; Ray Arden G. Demotica ; Anjunelle S. Esquivel ; Bryan E. Napuaga ; Chellie Mae D. Serrano and Maria Corina O. Velasco.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF _cJune 2017
300			_a64 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: Isolation and identification of certain genus and species of bacteria can be done through growth in a culture medium. One example of such medium is Chocolate Agar Plate (CAP), a non-selective media wherein certain enrichment factors are added to promote or enhance growth of some fastidious bacteria. Haemophilus influenzae are small, non-motile gram negative coccobacilli, and are fastidious by nature. They require factors for growth such as Heme factor and Nicotine Adenine Dinucleotide factor. These requirements are provide in CAP to support its growth. Theobroma cacao is a small evergreen tree that is readily available in the Philippines. In many studies, it was proven that cacao has inhibitory effects to bacteria but not the genus Haemophilus. This study involves the cultivation of a purer culture of H. influenzae through the replacement of the inhibitory agent bacitracin with crude extract of cocoa to see if the modified chocolate agar plate is capable of producing results equal to the standard or greater in terms of suppressing the growth of other contaminant bacteria thus growth of purer culture of H. influenzae. Theobroma cacao seed were ground and extracted via ethanolic extraction the subjected to rotary evaporation that yielded the crude extract and incorporated to the CAP as the experimental plates, Bacitracin CAP served as the control. Three concentrations are prepared from the Theobroma cacao crude extract. 4 organisms were used. Haemophilus influenza, as the organism in focus in the study and other organisms which are the usual undesired organisms. The organisms were inoculated on both the plates and observed for the growth and number of colonies. Results showed that modified CAP with seed extract from cacao tree with the concentration of 3% and 5% have not shown selectivity to H.influenzae while 10% of cacao modified CAP have not shown its selectivity but was able to show how increasing concentration of cocoa extract affect the growth of the selected organism.
521			_aTHMT
700			_aCalibuso, Wilniel Bryan F. _eauthor
700			_aCanoy, Ryan Kenneth G. _eauthor
700			_aCruz, Bernadette Jia M. _eauthor
700			_aCua, Anne Beatrize U. _eauthor
700			_aDedace, Bonn Joseph H. _eauthor
700			_aDemotica, Ray Arden G. _eauthor
700			_aEsquivel, Anjunelle S. _eauthor
700			_aNapuaga, Bryan E. _eauthor
700			_aSerrsno, Chellie Mae D. _eauthor
700			_aVelasco, Maria Corina O. _eauthor
942			_2lcc _cTH