000			03316nam a22003737a 4500
999			_c9974 _d9974
003			PILC
005			20240720152911.0
008			170726b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2017 0005
100			_aSherwin N. Reyes, RMT, MSc, ISID _eadviser
111			_dJune 2017
245			_aThe Capacity of Tobacco Extract in Inhibiting the Growth of Microorganisms In Vitro / _cJasmine Elaine G. Almasa ; Allyson Kim M. Bahala ; Jonel A. Bautista ; Lyra L Calub ; Sofia D. Libunao ; Gaibrielle B. Paican ; Kamille Mangubat ; Angelyn P. San Miguel ; Vanessa Claudine F. Tan ; Ariel Josiah C. Valencia and Kresamie A. Villar.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF _cJune 2017
300			_a31 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: According to the World Health Organization (WHO), the consumption of tobacco continues to be a major cause of health complications worldwide. However, nicotine in tobacco leaves, aside from its beneficial effects to nicotinophilic organisms, can also inhibit arrays of microorganisms. This can be used as an inhibitory agent in selective media since selective media frequently uses toxic substances as an inhibitory agent to select a specific bacteria to grow. In light of the studies about the capabilities of nicotine in tobacco extract as an enhancing agent for bacterial growth, the researchers pursued further studies about tobacco extract on its ability as an inhibiting agebt for bacterial growth in a broth medium. This was achieved by obtaining tobacco extract from Nicotiana tabacum L. ( Family Solanaceae) and incorporating it to a nutritive broth medium. After which, the researchers inoculated isolated bacteria provided by the FEU-NRMF microbiology laboratory on the prepared tobacco extract broth. This procedure was done to test the inhibiting capacity of the tobacco extract and to determine whether various factors such as concentration of tobacco extract, bacterial strain and incubation time affect the inhibitory effect. After the exposure of the organisms on the tobacco extract broth in different concentrations, only Staphylococcus aureus is resistant on the tobacco extract broth at even the highest concentration used (250 µg/100 µL) while Pseudomonas aeruginosa, Streptococcus pyogenes and Escherichia coli were inhibited depending on the concentration used. This study concluded that the higher the concentration of the tobacco extracts, the higher the inhibition of bacterial growth regardless of the strain and the incubation time.
521			_aTHMT
700			_aAlmasa, Jasmine Elaine G. _eauthor
700			_aBahala, Allyson Kim M. _eauthor
700			_aBautisa, Jonel A. _eauthor
700			_aCalub, Lyra L. _eauthor
700			_aLibunao, Sofia D. _eauthor
700			_aPaica, Gaibrielle B. _eauthor
700			_aMangubat, Kamille _eauthor
700			_aSan Miguel, Angelyn P. _eauthor
700			_aTan, Vanessa Claudine F. _eauthor
700			_aValencia, Ariel Josiah C. _eauthor
700			_aVillar, Kresamie A. _eauthor
942			_2lcc _cTH