000			03283nam a22003257a 4500
999			_c9995 _d9995
003			PILC
005			20240720152913.0
008			170729b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2017 0009
100			_aFlordeliza Gutierrez, RMT, RN, ASCPi, MPH _eadviser
111			_dJune 2017
245			_aComparison of Olive Oil and Canola Oil as a Substrate in Serum Lipase Determination / _cJoseph Anthony Mahmood A. Al-Tawalbeh ; Kate Alexis C. Fausto ; Anne Immaculate E. Go ; Jenny Rose G. Pascua ; Edgar jairus luigi T. Pile ; Dean D. Reyes ; Natalia Kristine F. Sandoval and Althea Lourese D.G. Sengson.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF _cJune 2017
300			_a40 pages: _billustrations, tables, photos, _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
520			_aAbstract: LIpase (EC 3.1.1.3) is one of the enzymes important in maintaining the homeostasis of the human body. Lipase performs essential roles in the processing of dietary lipids. Example of which is to hydrolyze triglycerides into their component fatty acid and glycerol molecules. Clinical chemistry assays for human lipase use serum as sample and are said to be exclusive to the diagnosis of acute pancreatitis. One method used for measuring the level of lipase in the serum uses a turbidimetric principle. This study has a primary purpose of comparing olive oil and canola oil when used as a substrate in the turbidimetric serum lipase determination. To do this, a comparison of the enzymatic activity of lipase to the two oil substrates was made. This study aims to prove whether canola oil can be used as a possible substrate in the turbidimetric method for serum lipase determination. An experimental research was conducted utilizing the use of human samples were taken from the door-to-door recruitment of healthy individuals ages 18-50 years old without history of any pancreatic liver diseases living around West Fairview, Quezon City. Simple random sampling was conducted through the use of the lottery method. The participants were screened and were given informed consent forms. Results and findings gathered were taken from the human samples that underwent the procedures for the turbidimetric assay for serum lipase determination. The results were then used to compare the enzymatic activity of lipase to the possible alternative substrate, canola oil and to the traditional substrate, olive oil. Paired sample t-test was used to compare the experimental results that were gathered. The results revealed that there is a statistically significant difference between the two oil substrate that were used. Therefore, Canola oil cannot be used as possible alternative substrate for serum lipase determination
521			_aTHMT
700			_aAl-Tawalbeh, Joseph Anthony Mahmood A. _eauthor
700			_aFausto, Kate Alexis C. _eauthor
700			_aGo, Anne Immaculate E. _eauthor
700			_aPascua, Jenny Rose G. _eauthor
700			_aPile, Edgar Jairus Luigi T. _eauthor
700			_aReyes, Dean D. _eauthor
700			_aSandoval, Natalia Kristine F. _eauthor
700			_aSengson, Althea Lourese D.G. _eauthor
942			_2lcc _cTH