000			03318nam a22003617a 4500
999			_c9997 _d9997
003			PILC
005			20240720152913.0
008			170729b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2017 0011
100			_aAlicia A. Aldave, RMT, MHPed, MSMT, MT(ASCPi) _eadviser
111			_dJune 2017
245			_aEffectiveness of Lyophilized Coconut Milk Based Agar as Fungal Base Medium / _cMariane Colisao ; Eunice Gem Dela Cruz ; Arlene Mae Dumallay ; Neil Gabriel Encina ; Lourdes Gayle Gamilde ; Marnelli Mari Manuel ; Beata May Olaivar ; Ma. Lorraine Olivas ; Natasha Elaine Pecache and May-Ann Tapiador.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF _cJune 2017
300			_a51 pages: _billustrations, tables, photos; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aAbstract: The gold standard for diagnosing an infection is culture and sensitivity. The general culture medium for fungi is Sabouraud's Dextrose Agar. Sabouraud's dextrose agar has an acidic pH which is conducive for the growth of fungi. The preparation and cost of Sabouraud's Dextrose Agar is both tedious and expensive. The researchers made use of commercially available lyophilized coconut milk because it contains certain amino acids, carbohydrates, and lipids which can be a growth factor when it is used as an ingredient for fungal culture medium. It also has an acidic pH which can act as natural antibacterial component. On the other hand, coconut milk is readily available in the market making it a feasible, practical and cost effective alternative for SDA. The study aimed to determine the effectiveness of lyophilized coconut milk based agar as a fungal base medium compared to Sabouraud's Dextrose agar (SDA) which is the general fungal media with the following criteria: Growth, Time of growth, Microscopic Morphological Features, Microscopic Morphological Features, and Cost Analysis. The experimental study design was used to evaluate coconut milk-based agar in culture media preparation and diagnosis of pathologically important fungi, together with Sadbouraud Dextrose Agar as the control for experimentation. The Two (2) media were subjected to different trials for comparison. For the preparation of coconut milk based agar the researchers used coconut milk powder (commercial sources)as a nutrient source in making the agar media, yeast was also added which acted as an additive source of carbohydrates and preservative agent and commercially prepared agar was used as a solidifying agent. The prepared lyophilized coconut milk based (LCMB) agar undergone pre heating autoclaving and dispensing onto the petri dish.
521			_aTHMT
700			_aColisao, Mariane _eauthor
700			_aDela Cruz, Eunice Gem _eauthor
700			_aDumallay, Arlene Mae _eauthor
700			_aEncina, Neil Gabriel _eauthor
700			_aGamilde, Lourdes Gayle _eauthor
700			_aManuel, Marnelli Mari _eauthor
700			_aOlaivar, Beata May _eauthor
700			_aOlivas, Ma. Lorraine _eauthor
700			_aPecache, Natasha Elaine _eauthor
700			_aTapiador, May-Ann _eauthor
942			_2lcc _cTH