000			03532nam a22003737a 4500
999			_c9999 _d9999
003			PILC
005			20240720152913.0
008			170729b xxu||||| |||| 00| 0 eng d
040			_cFEU-NRMF MEDICAL LIBRARY
050			_aMT 2017 0013
100			_aMr. Normel Aldave _eadviser
111			_dJune 2017
245			_aHylocereuscostaricencis (Dragon Fruit) Peel Extract as a Counterstain in Histological Staining / _cSheena Arrojo ; Lizette Anne Baratang :Alexis Pearl Despojo ; Jillan Libatique ; Clarisse Dale Gell Mallari ; Joi Esra Malundo ; Hatsumi Noda ; Justine Jaro Obligado ; Adrian Joseph Piasan ; Rhon Andrew Raneses and Isabella Chesca Tayag.
260			_aFairview, Quezon City _bSchool of Medical Technology, FEU-NRMF _cJune 2017
300			_a30 pages: _billustrations, tables, photos. _c28 cm.
336			_2rdacntent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes appendices and bibliographical references.
520			_aABSTRACT: Eosin, a synthetic dye that is used in routine hematoxylin and eosin (H&E) Staining technique in laboratories, has been reported to have effects on the human health and may potentially cause to the environment. This research aims to use natural products, specially the Dragon Fruit (Hylocereuspolyrhizus), to determine its efficacy as a counterstain. The dragon Fruit Extract will be obtained through a simple extraction and distillation method. Factors such as pH of the fruit, light, temperature and storage conditions will be taken into consideration as to preserve the color of the extract. The preparation of the tissue specimen, particularly the pork chop tissue undergone the routine procedure for tissue processing that uses paraffin and sectioning. The tissue staining procedure will follow the H&E Staining procedure for paraffin embedded sections. Twelve slides were used: nine of which were used to counterstain with Dragon Fruit Extract at different minutes; three other slides were used to counterstain with eosin. A Pathologist will evaluate the stained slides based on a criteria provided which includes: a) the degree of staining, b) the clarity of cellular components, and c) the intensity of the staining. The data collected was presented on a tabular form. Slides that were stained with Dragon fruit extract for 3 minutes and 5 minutes were graded as 3+ which is interpreted that the whole tissue was stained. Slides that were stained for 1 minute were graded 2+ which are interpreted as 50% of the tissue show staining color. In term clarity of the cellular components slides that were stained for 3 and 5 minutes were classified as clear thus, cellular components are visible. Slides that were stained for 1 minute were classified as not clear correlating the cellular components are not visible,. In terms of intensity, slides with 1 minute, 3 minute and 5 minutes were graded as contrasting meaning the stain differentiates cellular components from the rest to the tissue
521			_aTHMT
700			_aArrojo, Sheena _eauthor
700			_aBaratang, Lizette Anne _eauthor
700			_aDespojo, Alexis Pearl _eauthor
700			_aLibatique, Jillan _eauthor
700			_aMallari, Clarisse Dale Gell _eauthor
700			_aMalundo, Joi Esra _eauthor
700			_aNoda, Hatsumi _eauthor
700			_aObligado, Justine Jaro
700			_aPiasan, Adrian Joseph _eauthor
700			_aReneses, Rhon Andrew _eauthor
700			_aTayag, Isabella Chesca _eauthor
942			_2lcc _cTH