Lipid peroxidation activity and hepatic histology in carbon tetrachloride treated mice (mus musculus) supplemented with camellia sinensis (green tea) extracts / Medicine 1B2; Remedios P. Santos; Mari-Ann Bringas; Dolores V. Viliran.

Includes appendices and bibliographical references.

Abstract: In this study, crude extracts commercially available teas made from Camellia sinensis were assessed for their antioxidant properties in mice treated with 30% carbon tetrachloride (CCI4). The experimental set up was composed of 36 mice, grouped into 6. The mice in Groups I and II served as the negative control, and were intraperitoneally injected daily with phosphate buffer saline (PBS) and Corn Oil (CO) at doses of 50 ml/kg body weight and 25 ml/kg body weight, respectively. Mice in Group III served as the positive control, and was intraperitoneally injected with 30% CCL4 every 72 hours to induce hepatic injury and lipid perodixation. And finally, mice in Groups IV, V, and VI were intraperitoneally injected with the Camellia sinensis tea extracts at doses of 25 mg, 50 mg, and 75 mg/kg body weight. All groups injected with the tea extracts were also injected with CCI4 every 72 hours to induce hepatic injury. After 10 days, the mice were sacrificed and their liver tissues were excised and subjected to lipid peroxidation assay, protein determination assay, and preparation for histopathological studies. The results show that Group VI mice showed the lowest mean malondialdehyde (MDA) concentration at 0.07490 nmol MDA/mg protein, and Group III mice showed the highest MDA concentration at 0. 1033. Statistical analyses among the groups showed significant differences against the positive control, and the histopathological study conducted was able to support the lipid peroxidation assay results with the visual differences between the positive, negative controls, and the groups treated with the tea extracts.

Thesis - Department of Biochemistry & Nutrition