Commercial Bromelain as an Anticoagulant / Andre Vinzon I. Baldovino ; Mark Jeffrey C. Merced ; Audilyn P. Montano ; Venice Vernadette SJ. Nicolas ; Rommel Banjo Q. Pangan and Pia Karla S. Tempongko.

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Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF September 2013Description: 28 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2013 0002

Summary: Abstract: The goal of the study is to prove that bromelain has an anticoagulant property in vitro. Bromelain is an enzyme coming from the pineapple proven by other studies to have an anticoagulant effect. It possesses anticoagulant properties in vivo which reduce the ability of platelets to clump together, or aggregate, reports a paper published in the January-February 1999 issue of the medical journal "in Vivo" In the attempt to give a broader source of anticoagulant, the researchers thought of utilizing the commercialized pure and natural bromelain powder. The researchers determined the blood to anticoagulant ratio by means of trial and error method, using EDTA as the positive and whole blood as negative control. Bromelain powder was diluted to varying volumes of NSS to attain different anticoagulant concentrations. They determined the length of time of the anticoagulant activity of the bromelain by recording the time on which no clotting occurs after examining the presence and absence of clot formation. Then morphology was tested with the use of Wright and Giemsa staining technique. After subjecting specimens of blood to series of bromelain concentration the results showed anticoagulant activities that lasted from more than 24 minutes to less than 6 hours, but denied of positive findings due to use of EDTA as positive control. Also, the results showed that blood cells with bromelain powder were morphologically similar as that of blood cells using EDTA. Therefore the researchers concluded that the commercially prepared bromelain has an anticoagulant property. It had a longer anticoagulant activity than the negative control and no morphologic changes were noted in blood smear with bromelain as compared with EDTA. The blood cells with bromelain have the same appearance as that of EDTA.

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2013 0002 c.2 (Browse shelf(Opens below))	Available		T001570
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2013 0002 (Browse shelf(Opens below))	Available		T001111

Includes appendices and bibliographical references.

Abstract: The goal of the study is to prove that bromelain has an anticoagulant property in vitro. Bromelain is an enzyme coming from the pineapple proven by other studies to have an anticoagulant effect. It possesses anticoagulant properties in vivo which reduce the ability of platelets to clump together, or aggregate, reports a paper published in the January-February 1999 issue of the medical journal "in Vivo" In the attempt to give a broader source of anticoagulant, the researchers thought of utilizing the commercialized pure and natural bromelain powder. The researchers determined the blood to anticoagulant ratio by means of trial and error method, using EDTA as the positive and whole blood as negative control. Bromelain powder was diluted to varying volumes of NSS to attain different anticoagulant concentrations. They determined the length of time of the anticoagulant activity of the bromelain by recording the time on which no clotting occurs after examining the presence and absence of clot formation. Then morphology was tested with the use of Wright and Giemsa staining technique. After subjecting specimens of blood to series of bromelain concentration the results showed anticoagulant activities that lasted from more than 24 minutes to less than 6 hours, but denied of positive findings due to use of EDTA as positive control. Also, the results showed that blood cells with bromelain powder were morphologically similar as that of blood cells using EDTA. Therefore the researchers concluded that the commercially prepared bromelain has an anticoagulant property. It had a longer anticoagulant activity than the negative control and no morphologic changes were noted in blood smear with bromelain as compared with EDTA. The blood cells with bromelain have the same appearance as that of EDTA.

Thesis - School of Medical Technology

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