The Use of Eggshell (Gallus Galuss Domesticus) as a Source of Calcium Chloride (Caci2) Reagent for Partial Thromboplastin Time (PTT) /
The Use of Eggshell (Gallus Galuss Domesticus) as a Source of Calcium Chloride (Caci2) Reagent for Partial Thromboplastin Time (PTT) /
Neil Francis D. Bataller ; Jezreell John B. Batiola ; Patricia Erika S Cañares ; Deanne Joy M. Diaz ; Irish Mae T. Eslabra ; Arvin Joseph D.R. Gonzaga and Paula Marie L. Humilde.
- Fairview, Quezon City School of Medical Technology, FEU-NRMF 2017
- illustrations, tables, photos 28 cm.
Includes appendices and bibliographical references.
Abstract: Eggshell is the outer covering of an egg and mostly made up of calcium carbonate. Is insoluble to water, which makes the extraction difficult. Several researches found out that hydrochloric acid (HCI) can be used to extract the calcium chloride, which would be a significant material in blood clotting test such as Partial Thromboplastin time (PTT). The eggshells from household waste were boiled to remove unwanted materials and were pulvenized. Ten grams of calcium carbonate were mixed with 100 milliliters of 1M diluted HCI solution for 30 minutes. After hydrolysis, the residues were removed by filtration. The solution was heated to remove the water, which produced 9 grams of calcium chloride from eggshell's calcium carbonate. The extracted calcium chloride was weighed as 0.37 grams and was mixed with 100 milliliters of distilled water. The partial Thromboplastin Time Testing was done using Coagulizer™, a semi-automated coagulation machine. Descriptive statistics were used in order to compare the CaCl2 in eggshells and commercial CaCl2 in PTT determination. Shapiro-Wilk Test as used to validate the normality of the samples and "R Console" was used to see the results. Data from the testing of both reagents were gathered, processed on compared. Analysis showed a significant difference between the statistical means of the 2 reagents used. Normal samples tested by the commercial CaCl2 reagent produced normal results, while the reagent prepared from eggshells were outside the reference range. This study concluded that the CaCl2 reagent extracted from the eggshells is not as effective as the commercially prepared CaCl2 reagent due to the interference caused by the eggshell impurities and induce significantly different Partial Thromboplastin Time.
Thesis - School of Medical Technology
MT 2017 0020
Includes appendices and bibliographical references.
Abstract: Eggshell is the outer covering of an egg and mostly made up of calcium carbonate. Is insoluble to water, which makes the extraction difficult. Several researches found out that hydrochloric acid (HCI) can be used to extract the calcium chloride, which would be a significant material in blood clotting test such as Partial Thromboplastin time (PTT). The eggshells from household waste were boiled to remove unwanted materials and were pulvenized. Ten grams of calcium carbonate were mixed with 100 milliliters of 1M diluted HCI solution for 30 minutes. After hydrolysis, the residues were removed by filtration. The solution was heated to remove the water, which produced 9 grams of calcium chloride from eggshell's calcium carbonate. The extracted calcium chloride was weighed as 0.37 grams and was mixed with 100 milliliters of distilled water. The partial Thromboplastin Time Testing was done using Coagulizer™, a semi-automated coagulation machine. Descriptive statistics were used in order to compare the CaCl2 in eggshells and commercial CaCl2 in PTT determination. Shapiro-Wilk Test as used to validate the normality of the samples and "R Console" was used to see the results. Data from the testing of both reagents were gathered, processed on compared. Analysis showed a significant difference between the statistical means of the 2 reagents used. Normal samples tested by the commercial CaCl2 reagent produced normal results, while the reagent prepared from eggshells were outside the reference range. This study concluded that the CaCl2 reagent extracted from the eggshells is not as effective as the commercially prepared CaCl2 reagent due to the interference caused by the eggshell impurities and induce significantly different Partial Thromboplastin Time.
Thesis - School of Medical Technology
MT 2017 0020
