Conversion of Blood Type A to Blood Type O red cells by Endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum
Conversion of Blood Type A to Blood Type O red cells by Endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum
Anna Belen Alensuela M.D. & FEU-NRMF Medicine Section D Class of 2010-2011
- Fairview, Quezon City, Philippines Department of Biochemistry, FEU-NRMF 2011
- illustrations, tables , photos 28cm
Considering the fact that there is a high occurrence of hemolytic reactions due to the imbalance figures
between the supply and demand of blood (not only in the Philippines, but also worldwide), it is crucial to find
for an alternative that could prevent such unwanted incidences. This study primarily aimed to significantly
contnbute on the possibility of reducing the morbidity and mortality rates due to incorrect blood transfusion
and shoitage of blood supply by exploring the potential for the conversion of blood type A to O, which is
consideied the universal donor. This study focused on the use of Bifidobacterium longum, (obtained from
Pine Fiee
^e Dried Bifidobacterium powder, Pro-LSB food supplement tablets, and a commercially available
yogurt grown in a Bifidobacterium Selective Media (BSM) agar,) which contains an enzyme endo-alpha-Nacetylgalactosaminidase that may specifically convert blood type A red cells to blood type O. Each sample
from the three different sources was mixed with 5 drops of blood type A and tested for their effect. Each
prepared blood sample with the Bifidobacterium longum was incubated and checked for their agglutination
at an interval of 5 minutes, JOminutes, 60 minutes, 1,440 minutes (24 hours) and 2,880 minutes (48 hours).
The resulting blood type was determined through the use of an anti-sera and the absence of agglutination
after the reaction of the enzyme is indicative for blood Type O. The results showed that the longer the
bacteria and blood are exposed together, the agglutination decreases greatly from a strong agglutinate to a
weaker agglutinate. Though it has been observed that this idea can be conceivably developed further, it is
suggested by the researchers that more tests, experiments, variables and factors be considered to fully
determine its mechanism in converting a specific blood typ
Department of Biochemistry & Nutrition
M BIO 2011 0002
Considering the fact that there is a high occurrence of hemolytic reactions due to the imbalance figures
between the supply and demand of blood (not only in the Philippines, but also worldwide), it is crucial to find
for an alternative that could prevent such unwanted incidences. This study primarily aimed to significantly
contnbute on the possibility of reducing the morbidity and mortality rates due to incorrect blood transfusion
and shoitage of blood supply by exploring the potential for the conversion of blood type A to O, which is
consideied the universal donor. This study focused on the use of Bifidobacterium longum, (obtained from
Pine Fiee
^e Dried Bifidobacterium powder, Pro-LSB food supplement tablets, and a commercially available
yogurt grown in a Bifidobacterium Selective Media (BSM) agar,) which contains an enzyme endo-alpha-Nacetylgalactosaminidase that may specifically convert blood type A red cells to blood type O. Each sample
from the three different sources was mixed with 5 drops of blood type A and tested for their effect. Each
prepared blood sample with the Bifidobacterium longum was incubated and checked for their agglutination
at an interval of 5 minutes, JOminutes, 60 minutes, 1,440 minutes (24 hours) and 2,880 minutes (48 hours).
The resulting blood type was determined through the use of an anti-sera and the absence of agglutination
after the reaction of the enzyme is indicative for blood Type O. The results showed that the longer the
bacteria and blood are exposed together, the agglutination decreases greatly from a strong agglutinate to a
weaker agglutinate. Though it has been observed that this idea can be conceivably developed further, it is
suggested by the researchers that more tests, experiments, variables and factors be considered to fully
determine its mechanism in converting a specific blood typ
Department of Biochemistry & Nutrition
M BIO 2011 0002
