A Comparative study on the total phenolic content and scavenging activity of antioxidants from seeds of citrus microcarpa (calamansi), citrus sinensis (orange) and citrus aurantium (dalandan) /
A Comparative study on the total phenolic content and scavenging activity of antioxidants from seeds of citrus microcarpa (calamansi), citrus sinensis (orange) and citrus aurantium (dalandan) /
Section I-B; Joel Javate.
- Fairview, Quezon City: Department of Biochemistry, FEU-NRMF, 2011.
- 49 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Citrus fruits have good antioxidants properties which protect the body against radical attacks, balance glucose and cholesterol levels as well as reduce risk of heart diseases. These beneficial outcomes are attributed to flavonoids, in particular polyphenols, that scavenge free radicals and neutralize their damaging effect, inhibit hydrolytic and oxidative enzymes and prevent inflammation. Polyphenols are present in all parts of the fruit. Its seeds although containing these compounds are often regarded as a waste product and thus are not usually utilized for commercial purposes. This study aims to investigate on the antioxidant properties of three locally available citrus fruits namely, Citrus microcarpa (calamansi), Citrus sinensis (orange), and Citrus aurantium (dalandan). Folin-ciocalteau method and DPPH assay were employed to quantify the phenolic contents and antioxidant scavenging activity, respectively. Highest phenolic content expressed in Gallic Acid Equivalents (GAE), was observed in calamansi seed extract (45.020), followed by orange (31.832) and dalandan (29.00). Orange (89.190%) has the highest scavenging activity, followed by dalandan (83.398%), and calamansi (79.730%). Statistical analysis shows that the difference on total phenolic contents among the three fruits is significant (p value 0.000<0.05). However, difference on scavenging activities of the three fruits is insignificant (p value 0.73< 0.05). Only C. microcarpa showed a significant relationship between its scavenging activity and total phenolic content. It can be concluded based on the results that free radical scavenging activity may not be related to polyphenol levels even though these are present in the citrus seeds. Further evaluation of other antioxidant compounds that may have an effect on the free radical scavenging activity is recommended especially for calamansi.
Thesis - Department of Biochemistry & Nutrition
M BIO 2011 0001
Includes appendices and bibliographical references.
Abstract: Citrus fruits have good antioxidants properties which protect the body against radical attacks, balance glucose and cholesterol levels as well as reduce risk of heart diseases. These beneficial outcomes are attributed to flavonoids, in particular polyphenols, that scavenge free radicals and neutralize their damaging effect, inhibit hydrolytic and oxidative enzymes and prevent inflammation. Polyphenols are present in all parts of the fruit. Its seeds although containing these compounds are often regarded as a waste product and thus are not usually utilized for commercial purposes. This study aims to investigate on the antioxidant properties of three locally available citrus fruits namely, Citrus microcarpa (calamansi), Citrus sinensis (orange), and Citrus aurantium (dalandan). Folin-ciocalteau method and DPPH assay were employed to quantify the phenolic contents and antioxidant scavenging activity, respectively. Highest phenolic content expressed in Gallic Acid Equivalents (GAE), was observed in calamansi seed extract (45.020), followed by orange (31.832) and dalandan (29.00). Orange (89.190%) has the highest scavenging activity, followed by dalandan (83.398%), and calamansi (79.730%). Statistical analysis shows that the difference on total phenolic contents among the three fruits is significant (p value 0.000<0.05). However, difference on scavenging activities of the three fruits is insignificant (p value 0.73< 0.05). Only C. microcarpa showed a significant relationship between its scavenging activity and total phenolic content. It can be concluded based on the results that free radical scavenging activity may not be related to polyphenol levels even though these are present in the citrus seeds. Further evaluation of other antioxidant compounds that may have an effect on the free radical scavenging activity is recommended especially for calamansi.
Thesis - Department of Biochemistry & Nutrition
M BIO 2011 0001
