Glycine Max (Soy Bean) As Nutrient Agar /
Polly Chua-Chan, MD, MPH ; Alicia A. Aldave, RMT, MHPEd
Glycine Max (Soy Bean) As Nutrient Agar / Arvin P. Ayaay ; Nivard Vincent C. Babaran ; Renee Camile D. Balase ; Rafael Leonardo C. Ferrer ; Mary Gestine Gacot ; Angeli B. Guillermo ; Maricar Y. Pua ; Alesa Maureen M. Salcedo ; Renante P. Santos and Andrew Ronan F. Uy. - Fairview, Quezon City School of Medical Technology, FEU-NRMF September 2010 - 40 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Glycine max (Soy bean) is a legume rich in nutrients and components such as water, protein, fat, carbohydrate, crude fiber, calcium, iron, zinc, thiamine, riboflavin, niacin, vitamin B6, foliate, sugar, and sodium. Some of which are necessary for the replication of microorganisms. In this study, growth from a representative gram positive organism, Staphylococcus aureus and, from a representative gram negative gram negative organism, Eschericho coli on the Soy milk gelatin was compared to the standard Nutrient agar. The purpose was to create a similar nutrient agar capable of supporting, growth of non-fastidious organisms and is easier to produce, cost effective and has materials readily available. To compare the colony growth of the bacteria from both media, standards were used as a basis. The criteria used to characterize bacterial growth include colony size, chromogenicity, shape, surface appearance, visual characteristics, and changes in medium resulting from bacterial growth. The results showed that most of the colonies growing in the Nutrient Agar, 100% Soymilk Gelatin, and 50% Soymilk Gelatin produced the same characteristics after incubation period of 24, 48 and 72 hours. However, chromogenenicity was best exhibited on 50% Soymilk. The researchers recommend that the Soymilk Gelatin be tested as a chomogenic medium for S. aureus. In addition, the researchers recommend that it be compared to mannitol salt agar. Finally, the researchers recommend that NaCI be added to the medium to make it selective for S. aureus.
Thesis - School of Medical Technology
MT 2010 0006
Glycine Max (Soy Bean) As Nutrient Agar / Arvin P. Ayaay ; Nivard Vincent C. Babaran ; Renee Camile D. Balase ; Rafael Leonardo C. Ferrer ; Mary Gestine Gacot ; Angeli B. Guillermo ; Maricar Y. Pua ; Alesa Maureen M. Salcedo ; Renante P. Santos and Andrew Ronan F. Uy. - Fairview, Quezon City School of Medical Technology, FEU-NRMF September 2010 - 40 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Glycine max (Soy bean) is a legume rich in nutrients and components such as water, protein, fat, carbohydrate, crude fiber, calcium, iron, zinc, thiamine, riboflavin, niacin, vitamin B6, foliate, sugar, and sodium. Some of which are necessary for the replication of microorganisms. In this study, growth from a representative gram positive organism, Staphylococcus aureus and, from a representative gram negative gram negative organism, Eschericho coli on the Soy milk gelatin was compared to the standard Nutrient agar. The purpose was to create a similar nutrient agar capable of supporting, growth of non-fastidious organisms and is easier to produce, cost effective and has materials readily available. To compare the colony growth of the bacteria from both media, standards were used as a basis. The criteria used to characterize bacterial growth include colony size, chromogenicity, shape, surface appearance, visual characteristics, and changes in medium resulting from bacterial growth. The results showed that most of the colonies growing in the Nutrient Agar, 100% Soymilk Gelatin, and 50% Soymilk Gelatin produced the same characteristics after incubation period of 24, 48 and 72 hours. However, chromogenenicity was best exhibited on 50% Soymilk. The researchers recommend that the Soymilk Gelatin be tested as a chomogenic medium for S. aureus. In addition, the researchers recommend that it be compared to mannitol salt agar. Finally, the researchers recommend that NaCI be added to the medium to make it selective for S. aureus.
Thesis - School of Medical Technology
MT 2010 0006
