Disinfectant Properties of Balete (Ficus Benjamina Linn.) Stem and Bark Extract /
Sherwin N. Reyes, RMT
Disinfectant Properties of Balete (Ficus Benjamina Linn.) Stem and Bark Extract / Gladys G. Abo ; Alejandro I. Aguilar III ; Charmaine E. Asehan ; Pia Shiela B. Badong ; Ria Shekina B. Lumbao ; Ma. Rosalina F. Mendres ; Allan Henry P. Nadong ; Marby Xyra A. Perez and Jamille Turingan. - Fairview, Quezon City School of Medical Technology, FEU-NRMF - 30 pages: illustrations, tables; 28 cm.
Includes bibliographical references.
Abstract: Ficus benjamina, commonly known as weeping fig, benjamin's fig, or simply ficus, is an evergreen epiphytic tree under the family of moraceae. Ficus is native to a large area including india, southern china, southeast asia, malaysia, the philippines, northern australia, and the islands of the south pacific. This epiphytic tree is well known for its medical potential. The plant can be used as a treatment for certain disorders, antinociceptive, antipyretic, stomachic, hypotensive and anti-dysentery remedy. The ficus species have been reported to have enormous phytochemical constituents including tannins, flavonols, terpenoids, coumarins, glycosides, esters, carbohydrates, serine protease, and others. This research is an experimental study which examined the disinfectant properties of the stem and bark extract of ficus benjamina linn against the experimental bacteria used: staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli. It was obtained by gathering young stem and bark by processing it to obtain the extract. The bacterial growth of the disinfectant was determined using the rideal walker method. Test disinfectant was diluted from 1;400 to 1:800 and the phenol was diluted from 1:95 to 1:115. Their bactericidal activity was determined against staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli suspension. Subcultures are performed from both the test and phenol at intervals of 2.5, 5, 7.5 and 10 minutes. The plates are incubated for 48-72 hours at 37 c. The dilution of disinfectant which disinfectant which disinfects the suspension in given time is divided by that dilution of phenol which disinfects the suspension in same time gives its phenol coefficient. Phytochemical analysis showed the presence of alkaloids, condensed tannins, 2 deoxysugar, unsaturated steroids and triterpenes, flavonoids or y-benzopyrone nucleus, fats and oils. The antimicrobial potency of the plant may be attributed to single or combined effect of the mentioned chemical groups. The role of these constituents in eliciting antimicrobial properties require further detailed phytochemical investigation. From the observations made, the extracts from the stem and bark of ficus benjamina were found to show very weak antimicrobial activity when compared to the standard disinfectant (lysol). Although the extract of the plant did not show complete inhibition (no growth on the agar plates), it showed visible reduction in the number of colonies of bacteria as the concentration of the extract increased. The study shows that increasing the concentration of the balete extract further will increase the inhibitory activity of the antimicrobial of the extract. It was also evident that as the time of exposure to the extract increased, stronger inhibitory activity was observed especially with the number of colonies on the agar plates.
Thesis - Department of Biochemistry & Nutrition
MT ND 0005
Disinfectant Properties of Balete (Ficus Benjamina Linn.) Stem and Bark Extract / Gladys G. Abo ; Alejandro I. Aguilar III ; Charmaine E. Asehan ; Pia Shiela B. Badong ; Ria Shekina B. Lumbao ; Ma. Rosalina F. Mendres ; Allan Henry P. Nadong ; Marby Xyra A. Perez and Jamille Turingan. - Fairview, Quezon City School of Medical Technology, FEU-NRMF - 30 pages: illustrations, tables; 28 cm.
Includes bibliographical references.
Abstract: Ficus benjamina, commonly known as weeping fig, benjamin's fig, or simply ficus, is an evergreen epiphytic tree under the family of moraceae. Ficus is native to a large area including india, southern china, southeast asia, malaysia, the philippines, northern australia, and the islands of the south pacific. This epiphytic tree is well known for its medical potential. The plant can be used as a treatment for certain disorders, antinociceptive, antipyretic, stomachic, hypotensive and anti-dysentery remedy. The ficus species have been reported to have enormous phytochemical constituents including tannins, flavonols, terpenoids, coumarins, glycosides, esters, carbohydrates, serine protease, and others. This research is an experimental study which examined the disinfectant properties of the stem and bark extract of ficus benjamina linn against the experimental bacteria used: staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli. It was obtained by gathering young stem and bark by processing it to obtain the extract. The bacterial growth of the disinfectant was determined using the rideal walker method. Test disinfectant was diluted from 1;400 to 1:800 and the phenol was diluted from 1:95 to 1:115. Their bactericidal activity was determined against staphylococcus aureus, pseudomonas aeruginosa, and escherichia coli suspension. Subcultures are performed from both the test and phenol at intervals of 2.5, 5, 7.5 and 10 minutes. The plates are incubated for 48-72 hours at 37 c. The dilution of disinfectant which disinfectant which disinfects the suspension in given time is divided by that dilution of phenol which disinfects the suspension in same time gives its phenol coefficient. Phytochemical analysis showed the presence of alkaloids, condensed tannins, 2 deoxysugar, unsaturated steroids and triterpenes, flavonoids or y-benzopyrone nucleus, fats and oils. The antimicrobial potency of the plant may be attributed to single or combined effect of the mentioned chemical groups. The role of these constituents in eliciting antimicrobial properties require further detailed phytochemical investigation. From the observations made, the extracts from the stem and bark of ficus benjamina were found to show very weak antimicrobial activity when compared to the standard disinfectant (lysol). Although the extract of the plant did not show complete inhibition (no growth on the agar plates), it showed visible reduction in the number of colonies of bacteria as the concentration of the extract increased. The study shows that increasing the concentration of the balete extract further will increase the inhibitory activity of the antimicrobial of the extract. It was also evident that as the time of exposure to the extract increased, stronger inhibitory activity was observed especially with the number of colonies on the agar plates.
Thesis - Department of Biochemistry & Nutrition
MT ND 0005
