Isolation, Identification and Resistogram of Bacterial Consortia mong Food Handlers /
Sherwin N. Reyes, RMT
Isolation, Identification and Resistogram of Bacterial Consortia mong Food Handlers / Princess Era Grace K. Adao ; Nadynne Mari C. Cauli ; Barty B. Castro IV ; Daniel John Lorette H. Dela Cruz ; Airam Camille N. Malilay ; Genevieve Lynette C. Maylem ; Jonathan G. Millora ; Mikaela Marie N. Nuval and John Francis Klintone D. Orgas. - Fairview, Quezon City School of Medical Technology, FEU-NRMF 2016 - 70 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Street foods consist a wide range of ready-to-eat foods and beverages sold and sometimes prepared in public places. It is convenient, fast, easy, uncomplicated, and cheap. However, they have some potential public health risks associated with their preparation and consumption which could promote bacterial overgrowth and contamination, increasing the hazards for the consumers. This study aims to investigate the health and safety issues in food handling and preparation among food handlers surrounding Far Eastern University dr. Nicanor Reyes Medical Foundation, Fairview Campus, Quezon City. A descriptive cross-sectional study was conducted among food handlers within the vicinity of Far Eastern University - Dr. Nicanor Reyes Medical Foundation (FEU-NRMF) at Barangay West Fairview, Quezon City. There were forty four (44) food handlers participated in the study. The socio demographic profile was collected using a structured questionnaire and hand swab samples was collected from participants and microbiologically examined for the presence of potential food borne bacterial contaminants using standard laboratory techniques and methods. The data was summarized in descriptive statistical methods and chi-square test was employed to check for any possible association between variables. Among the forty four (44) food handlers identified and tested, 39 (88.6%) tested positive for the presence of potential food borne pathogens. Majority of the food handlers were female, 26 (59.09%). Kleibsella spp. 20 (51.28%) was the predominant bacterial specie followed by pseudomonas aeruginosa 5 (12.82%), and staphylococcus aureus 4 (10.26%). S. Aureus isolates showed resistance to penicillin, oxacillin and clindamycin and no s. Aureus isolate showed resistance to cefoxitin. P. Aeruginosa showed resistance to Azteronam, Amoxiclav-Clavulanate, Cefotaxime and Cefoxitin No Kleibsella spp. Isolated showed resistance to cefepime and imipenem. Isolation of kleibsella spp., s. Aureus and other public health important bacteria from food handlers stressed that the hands of food handlers serves as a potential vector of food borne bacterial pathogens that could pose a potential risks of food borne disease outbreaks. Therefore, promotion of proper hand washing, training on personal hygiene, and assessment of hygiene management and facilities as well as regulation of government policies and standards should be considered and strictly implemented.
Thesis - School of Medical Technology
MT 2016 0004
Isolation, Identification and Resistogram of Bacterial Consortia mong Food Handlers / Princess Era Grace K. Adao ; Nadynne Mari C. Cauli ; Barty B. Castro IV ; Daniel John Lorette H. Dela Cruz ; Airam Camille N. Malilay ; Genevieve Lynette C. Maylem ; Jonathan G. Millora ; Mikaela Marie N. Nuval and John Francis Klintone D. Orgas. - Fairview, Quezon City School of Medical Technology, FEU-NRMF 2016 - 70 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: Street foods consist a wide range of ready-to-eat foods and beverages sold and sometimes prepared in public places. It is convenient, fast, easy, uncomplicated, and cheap. However, they have some potential public health risks associated with their preparation and consumption which could promote bacterial overgrowth and contamination, increasing the hazards for the consumers. This study aims to investigate the health and safety issues in food handling and preparation among food handlers surrounding Far Eastern University dr. Nicanor Reyes Medical Foundation, Fairview Campus, Quezon City. A descriptive cross-sectional study was conducted among food handlers within the vicinity of Far Eastern University - Dr. Nicanor Reyes Medical Foundation (FEU-NRMF) at Barangay West Fairview, Quezon City. There were forty four (44) food handlers participated in the study. The socio demographic profile was collected using a structured questionnaire and hand swab samples was collected from participants and microbiologically examined for the presence of potential food borne bacterial contaminants using standard laboratory techniques and methods. The data was summarized in descriptive statistical methods and chi-square test was employed to check for any possible association between variables. Among the forty four (44) food handlers identified and tested, 39 (88.6%) tested positive for the presence of potential food borne pathogens. Majority of the food handlers were female, 26 (59.09%). Kleibsella spp. 20 (51.28%) was the predominant bacterial specie followed by pseudomonas aeruginosa 5 (12.82%), and staphylococcus aureus 4 (10.26%). S. Aureus isolates showed resistance to penicillin, oxacillin and clindamycin and no s. Aureus isolate showed resistance to cefoxitin. P. Aeruginosa showed resistance to Azteronam, Amoxiclav-Clavulanate, Cefotaxime and Cefoxitin No Kleibsella spp. Isolated showed resistance to cefepime and imipenem. Isolation of kleibsella spp., s. Aureus and other public health important bacteria from food handlers stressed that the hands of food handlers serves as a potential vector of food borne bacterial pathogens that could pose a potential risks of food borne disease outbreaks. Therefore, promotion of proper hand washing, training on personal hygiene, and assessment of hygiene management and facilities as well as regulation of government policies and standards should be considered and strictly implemented.
Thesis - School of Medical Technology
MT 2016 0004
