Commercially Prepared Lyophilized Lycopene Extract as a Histopathologic Counterstain for Tissue Samples /
Dr. Jocelyn Q. Gacasan
Commercially Prepared Lyophilized Lycopene Extract as a Histopathologic Counterstain for Tissue Samples / Almira Isabel Clarito ; Jeremy Anne Cruz ; Sarah Patrice Go ; Miya Lu ; Ryan Jasper Ngo ; Mary Ernestine Nuguid ; Vince Anthony Tirol and Meredith Valdez. - Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017 - 82 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: A patient's welfare is very medical professional's priority. Hence, the field is always open to advancements and discoveries that could provide better. Our research divulges lycopene's potential to be a histopathologic counterstain for tissue samples. This is in response to eosin's issue om stain leaching, as well as to exploit the copious amount of organic dye-yielding plants. Histopathological staining play an important role in the diagnosis of diseases and one of the credited gold standards is the H&E staining. However, eosin leaching has been evident and may be attributed to its solubility in water. Thus, a dye which is water insoluble such as lycopene will be put into test if it can stain cytoplasm, collagen and muscles with the right intensity of color as much as eosin would. The study was conducted assuming that indeed, lycopene can be a histopathological satin. The subject was a processed tissue of a lean meat from pig, this undergone H&E staining with eosin as the control and with lycopene as the variable. With this, a comparison was made according to its ability to stain and the color intensity produced by the stain. Ability to satin will be evaluated by whether it will be able to stain or not. On the other hand, color intensity will be defined as understained, sufficiently stained or overstained. This experimental study made use of the Chi Square test as the statistical tool to determine the significance of the hypothesis. After examining 40 tissue slides, 20 of which was stained by eosin and the other 20 stained by lycopene that was evaluated by a pathologist, the data gathered brought in a good vision for lycopene to be alternative strain. Focusing on the ability strain, lycopene is comparable to eosin. On the contrary however, regarding the color intensity produced, lycopene is not comparable to eosin as it yielded undrstrained tissue samples.
Thesis - School of Medical Technology
MT 2017 0008
Commercially Prepared Lyophilized Lycopene Extract as a Histopathologic Counterstain for Tissue Samples / Almira Isabel Clarito ; Jeremy Anne Cruz ; Sarah Patrice Go ; Miya Lu ; Ryan Jasper Ngo ; Mary Ernestine Nuguid ; Vince Anthony Tirol and Meredith Valdez. - Fairview, Quezon City School of Medical Technology, FEU-NRMF June 2017 - 82 pages: illustrations, tables, photos; 28 cm.
Includes appendices and bibliographical references.
Abstract: A patient's welfare is very medical professional's priority. Hence, the field is always open to advancements and discoveries that could provide better. Our research divulges lycopene's potential to be a histopathologic counterstain for tissue samples. This is in response to eosin's issue om stain leaching, as well as to exploit the copious amount of organic dye-yielding plants. Histopathological staining play an important role in the diagnosis of diseases and one of the credited gold standards is the H&E staining. However, eosin leaching has been evident and may be attributed to its solubility in water. Thus, a dye which is water insoluble such as lycopene will be put into test if it can stain cytoplasm, collagen and muscles with the right intensity of color as much as eosin would. The study was conducted assuming that indeed, lycopene can be a histopathological satin. The subject was a processed tissue of a lean meat from pig, this undergone H&E staining with eosin as the control and with lycopene as the variable. With this, a comparison was made according to its ability to stain and the color intensity produced by the stain. Ability to satin will be evaluated by whether it will be able to stain or not. On the other hand, color intensity will be defined as understained, sufficiently stained or overstained. This experimental study made use of the Chi Square test as the statistical tool to determine the significance of the hypothesis. After examining 40 tissue slides, 20 of which was stained by eosin and the other 20 stained by lycopene that was evaluated by a pathologist, the data gathered brought in a good vision for lycopene to be alternative strain. Focusing on the ability strain, lycopene is comparable to eosin. On the contrary however, regarding the color intensity produced, lycopene is not comparable to eosin as it yielded undrstrained tissue samples.
Thesis - School of Medical Technology
MT 2017 0008
