MARC details
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03995nam a22004697a 4500 |
| 001 - CONTROL NUMBER |
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MT 2023 0003 |
| 003 - CONTROL NUMBER IDENTIFIER |
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PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20240720153315.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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230524b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Language of cataloging |
eng |
| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| Description conventions |
rda |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
english |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
MT 2023 0003 |
| 245 ## - TITLE STATEMENT |
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| Title |
The Utilization of dye extracted from hylocereus costaricensis (red dragon fruit) as a counterstain in gram staining / |
| Statement of responsibility, etc. |
Joyce C. Ador Dionisio, Danycah Amor H. Amorio, Julia Mae R. Atega, Alyssa Baquir, Lourise Joyce V. Carreon, Joshua A. Carubanga, Alessandra Gabrielle M. Cristobal, Janiea G. Del Rosario, Cheyenne Kaye P. Espiritu, Lara Gwyneth M. Padamada and Martin Lawrence M. Punzal. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City: |
| Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF, |
| Date of publication, distribution, etc. |
2023. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
93 pages: |
| Other physical details |
illustrations, photos, tables; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Abstract: Environmental awareness has changed toward using naturally sourced color pigments in food items as alternatives for synthetic counterparts due to the growing toxicity of dyes employed in laboratories. One of those synthetic dyes that seriously irritates the eyes as well as the skin is safranin, a counterstain used in the Gram staining method. In this study, the researchers investigated the staining capability of the Hylocereus costaricensis (red dragon fruit) dye as a counterstain to Staphylococcus aureus and Escherichia coli, which is known gram-positive and gram-negative bacteria respectively using the routine Gram staining procedure. Two experiments were conducted as part of this research. Both experiments use the same extraction method, which is a crude extract, with the exception of the materials, the amount of Hylocereus costaricensis (red dragon fruit), and the solvents that were employed. The first experiment only used methanol as a solvent and Hylocereus costaricensis (red dragon fruit) peels to extract the dye. In contrast, in the second experiment, both the flesh and the peel of the Hylocereus costaricensis (red dragon fruit) were employed, and in addition to methanol, ethanol was used separately as a solvent. In order to extract the dye and test its staining capability, an experimental method was used. The staining potential of Hylocereus costaricensis (red dragon fruit) dye was then assessed using Gram staining method. The extracted dye extracts from both experiments showed an acidic pH, which ranged from 4.4 to 4.9. in each of the experimental samples, the researchers found that none of the extracted dye concentrations could stain the gram-negative bacteria (Escherichia coli) as evidenced by the fact that the stain obtained from Hylocereus costaricensis (red dragon fruit) did not adhere to the bacteria cell walls microscopically. As a result, the researchers concluded that the Hylocereus costaricensis (red dragon fruit) dye using a crude extraction method cannot be employed as a counterstrain in the Gram staining procedure. |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - School of Medical Technology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
synthetic dyes |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
safrain |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
gram staining |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
hylocereus costaricensis (red dragon fruit) |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
gram-positive bacteria |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
gram-negative bacteria |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
staphylococcus aureus |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
escherichia coli |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ador Dionisio, Joyce C. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Amorio, Danycah Amor H. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Atega, Julia Mae R. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Baquir, Alyssa |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Carreon, Lourise Joyce V. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Carubanga, Joshua A. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cristobal, Alessandra Gabrielle M. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Del Rosario, Janiea G. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Espiritu, Cheyenne Kaye P. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Padamada, Lara Gwyneth M. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Punzal, Martin Lawrence M. |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Room Use |
