: A comparative study between glacial acetic acid and citrofortunella aurantifolia (key lime) extract as white blood cell diluting fluid in leukemic blood samples (Record no. 13150)

MARC details
000 -LEADER
fixed length control field 03558nam a22003737a 4500
001 - CONTROL NUMBER
control field T002881
003 - CONTROL NUMBER IDENTIFIER
control field PILC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20241028103154.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 241028b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Language of cataloging eng
Transcribing agency FEU-NRMF MEDICAL LIBRARY
Modifying agency rda
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number MT 2024 0025 c.1
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Acosta, Marier Divine L.,
Relator term author
245 01 - TITLE STATEMENT
Title : A comparative study between glacial acetic acid and citrofortunella aurantifolia (key lime) extract as white blood cell diluting fluid in leukemic blood samples
Remainder of title [author]Acosta, Marier Divine L., Arias, Shaira Angela B., Bringas, Manuel Alexander II B., Esguerra, John Leuvin C. Grande, Allyssa Marie B., Licup, Roni Margaret N., Macapallag, Maria Amor, Maningas, Andrea Beatrice A., Mendoza, Ma. Andrea Dominique L., Nava, Zoe Maree D., Perez, John Renmar C.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Quezon City, Philippines,
Name of publisher, distributor, etc. FEU-NRMF Dr. Nicanor Reyes Medical Foundation
Date of publication, distribution, etc. 2024
300 ## - PHYSICAL DESCRIPTION
Extent 112 pages
Dimensions 28cm
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc Includes Appendix
520 ## - SUMMARY, ETC.
Summary, etc. ABSTRACT:<br/>Accurate white blood cell (WBC) counting lies in establishing a definitive WBC diluting fluid. The study undertakes a comparative analysis between glacial acetic acid, an established WBC diluting fluid, and Citrofortunella aurantifolia (key lime) extract in terms of their ability to lyse red blood cells (RBCs), preserve WBC visibility, and facilitate WBC counting. The participants' blood samples were divided into two: a control group (healthy individuals), and an experimental group (a patient diagnosed with leukemia). The key lime extract was subjected to a dilution process with normal saline solution (NSS) in three different ratios - 1:2, 1:3, and 1:4. The key lime and NSS solution were centrifuged at 5,000 rpm for 5 minutes, collecting the supernatant as the diluting fluid. The blood samples collected from participants were treated with C. aurantifolia diluting fluid and commercially prepared 2% glacial acetic acid. The samples were examined by two registered medical technologists using a counting chamber and graded according to their efficiency of RBC lysis, WBC visibility, and WBC count to assess both diluting<br/>fluids. A1:2 dilution of key lime extract (lmL key lime extract: 2mL NSS) demonstrated nearly the same effect as glacial acetic acid. The researchers concluded that key lime extract was comparable to glacial acetic acid in preserving WBC visibility and facilitating WBC count. However, it was not as efficient as commercially prepared glacial acetic acid, particularly in the<br/>lysis of RBCs. Further, the efficacy is limited only for samples with blood counts within normal range, and not for leukemic samples due to its high WBC count.<br/>Keywords: Blood Cell Analysis, Hematology, Red Blood Cell Lysis, White Blood Cell Counting<br/>
521 ## - TARGET AUDIENCE NOTE
Target audience note School of Medical Technology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Arias, Shaira Angela B.,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Bringas, Manuel Alexander II B.,
Relator term authos
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Esguerra, John Leuvin C.
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Grande, Allyssa Marie B
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Licup, Roni Margaret N.,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Macapallag, Maria Amor,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Maningas, Andrea Beatrice A.,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Mendoza, Ma. Andrea Dominique L.,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Nava, Zoe Maree D.,
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Perez, John Renmar C.
Relator term author
856 ## - ELECTRONIC LOCATION AND ACCESS
Enable Ebook Yes
Text to speech No
Electronic format type pdf
Uniform Resource Identifier <a href="https://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=7757a64b614ac976f71ca225e745c53c">https://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=7757a64b614ac976f71ca225e745c53c</a>
Link text Click here for FULL TEXT
Permission ALL
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/28/2024   MT 2024 0025 c.1 T002880 10/28/2024 10/28/2024 Theses
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/28/2024   MT 2024 0025 c.2 T002881 10/28/2024 10/28/2024 Theses
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/28/2024   MT 2024 0025 c.3 T002882 10/28/2024 10/28/2024 Theses