Citrus maxima (Pomelo) D- limonene volatile oil extract as a deparaffinization agent in histopathology (Record no. 13165)

MARC details
000 -LEADER
fixed length control field 03139nam a22003017a 4500
001 - CONTROL NUMBER
control field T002907
003 - CONTROL NUMBER IDENTIFIER
control field PILC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20241030130233.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 241030b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Language of cataloging eng
Transcribing agency FEU-NRMF MEDICAL LIBRARY
Modifying agency rda
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number MT 2024 0033 c.1
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Comaling, Carl Allen M.
Relator term author
245 01 - TITLE STATEMENT
Title Citrus maxima (Pomelo) D- limonene volatile oil extract as a deparaffinization agent in histopathology
Remainder of title [author]: Carl Allen M. Comaling, Chelsee Yvhon G. Julo, Cris May Bernadette A. Limbaga, Lyca R. Lumaad, Gwyneth Ann P. Masucol, Mark Alexander V. Umipig
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Quezon City, Philippines;
Name of publisher, distributor, etc. FEU-NRMF Dr. Nicanor Reyes Medical Foundation Institute of Medicine,
Date of publication, distribution, etc. 2024
300 ## - PHYSICAL DESCRIPTION
Extent 76pages
Dimensions 28cm
336 ## - CONTENT TYPE
Source rdacontext
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc Includes Appendix
520 ## - SUMMARY, ETC.
Summary, etc. ABTRACTS: <br/>INTRODUCTION: Xylene is commonly used in laboratories, primarily in histopathology, in order to enhance the transparency of tissue slides. However, it posed significant risks, including flammability and adverse health effects from inhalation or skin exposure. Consequently, the research investigated alternative substances, such as D-limonene<br/>METHOD: Data were collected from observation made by pathologists, employing a methodology that involved manipulating a single variable to examine its effect on another over a nine-month period. The data were organized into a table, and the Mann-Whitney U Test was used to assess the significance and efficacy of the deparaffinization agents. This test was carried out with IBM SPSS, and the p-value was set at 0.05. A pathologist evaluated the parameters using a scoring criterion. The Citrus maxima volatile extract was first obtained using steam distillation and then concentrated through evaporation.<br/>RESULT: The study revealed that the volatile oil, which includes D-limonene, efficiently deparaffinized tissue specimens while Keeping the integrity. The Treated slides were assessed as good to excellent, with a few variations supporting their diagnostic relevance. Based on its efficacy in deparaffinization, Staining quality, and cell morphology preservation, Citrus maxima (Pomelo) D-limonene volatile oil extract appears to be a feasible and perhaps less hazardous clearing agent compared to xylene for tissue processing histopathology.<br/>CONCLUSION: The study emphasized the necessity of investigating less hazardous deparaffinization methods, providing useful insights for medical technology, education, and lowering the health-associated risk with standard reagents such as xylene.<br/>KEYWORDS: Xylene, Mann-Whitney U Test, IBM SPSS, volatile, Steam Distillation evaporation<br/><br/>
521 ## - TARGET AUDIENCE NOTE
Target audience note School of Medical Technology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Julo, Chelsee Yvhon G.
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Limbaga, Cris May Bernadette A.
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Masucol, Gwyneth Ann P.
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Umipig Mark Alexander V.
Relator term author
856 ## - ELECTRONIC LOCATION AND ACCESS
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Text to speech No
Electronic format type pdf
Uniform Resource Identifier <a href="https://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=b657e41b3148434776fee4f01b775ed1">https://library.feu-nrmf.ph/cgi-bin/koha/opac-retrieve-file.pl?id=b657e41b3148434776fee4f01b775ed1</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/30/2024   MT 2024 0034 c.1 T002907 10/30/2024 10/30/2024 Theses
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/30/2024   MT 2024 0034 c.2 T002908 10/30/2024 10/30/2024 Theses
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation 10/30/2024   MT 2024 0034 c.3 T002909 10/30/2024 10/30/2024 Theses