THE ROLE OF SQMI PROTEIN IN METHOTREXATE TRANSPORT AND SENSITIVITY OF HUMAN CARCINOMAS (Record no. 8640)

MARC details
000 -LEADER
fixed length control field 02264nam a22001937a 4500
003 - CONTROL NUMBER IDENTIFIER
control field PILC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240720152650.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160505b1997 xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency FEU-NRMF MEDICAL LIBRARY
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title English
050 ## - LIBRARY OF CONGRESS CALL NUMBER
Classification number FMBIO19970001
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name De Villa, Romulo-Jacinto Sales
245 ## - TITLE STATEMENT
Title THE ROLE OF SQMI PROTEIN IN METHOTREXATE TRANSPORT AND SENSITIVITY OF HUMAN CARCINOMAS
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Quezon City
Name of publisher, distributor, etc. FEU-NRMF
Date of publication, distribution, etc. April 1997
300 ## - PHYSICAL DESCRIPTION
Extent 197 pages
520 ## - SUMMARY, ETC.
Summary, etc. ABSTRACT: The levels of a novel membrane protein, SQMI, has been found to correlate with sensitivity of uncloned aquamous carcinoma cell lines to methotrexate (MTX) (Bernal et. al., 1990). This dissertation research was done to identify the mechanism of MTX resistence correlated with SQMI protein levels and to understand the role of SQMI protein in MTX sensitivity. Primary cultures and uncloned cell lines of various lung carcinoma cell types show a correlation of MTX sensitivity with SQMI protein levels and MTX transpoert indicating a possible role of SQMI protein in MTX sentivity by increased MTX transport SQMI protein levels also correlated with the degree of differentiation of the lung cancer cell type. In bronchial epithelial cells induced toward squamous differentiation, SQMI protein was transiently induced during early stages of squamous differentiation of NBE cells concomitant with increased MTX sensitivity. MTX resistent cloned cell lines were developed in order to pin point the mechanism of resistance that correlates with SQMI protein levels. MTX resistant sublines developed from a clonally derived SqCHN cell line, SCC15S1, showed high correlation of SQMI protein expression with MTX transport but not with other mechanism of MTX resistence such as MTX-polyglutamylation and DHFR enzyme activity. Exposure of MTX resistent SCC15R1.3 to SQMI protein in the media led to increased MTX sensitivity and transport. Exposure of MTX resistant cells to liposomes containing SQM1 protein also increased MTX uptake and sensitivity. Transfection of SQM1 cDNA in a mammalian expression vector, p205, again increased MTX uptake and sensitivity of CEM lymphoblastic lymphoma cells and MTX resistant SCC1R1.3.
521 ## - TARGET AUDIENCE NOTE
Target audience note Department of Biochemistry
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
    Library of Congress Classification     Far Eastern University - Nicanor Reyes Medical Foundation Far Eastern University - Nicanor Reyes Medical Foundation Theses 02/11/2015   FMBIO19970001 T000729 02/11/2015 07/22/2016 Theses