THE ROLE OF SQMI PROTEIN IN METHOTREXATE TRANSPORT AND SENSITIVITY OF HUMAN CARCINOMAS (Record no. 8640)
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fixed length control field | 02264nam a22001937a 4500 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | PILC |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20240720152650.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 160505b1997 xxu||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE | |
Transcribing agency | FEU-NRMF MEDICAL LIBRARY |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | English |
050 ## - LIBRARY OF CONGRESS CALL NUMBER | |
Classification number | FMBIO19970001 |
100 ## - MAIN ENTRY--PERSONAL NAME | |
Personal name | De Villa, Romulo-Jacinto Sales |
245 ## - TITLE STATEMENT | |
Title | THE ROLE OF SQMI PROTEIN IN METHOTREXATE TRANSPORT AND SENSITIVITY OF HUMAN CARCINOMAS |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Place of publication, distribution, etc. | Quezon City |
Name of publisher, distributor, etc. | FEU-NRMF |
Date of publication, distribution, etc. | April 1997 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | 197 pages |
520 ## - SUMMARY, ETC. | |
Summary, etc. | ABSTRACT: The levels of a novel membrane protein, SQMI, has been found to correlate with sensitivity of uncloned aquamous carcinoma cell lines to methotrexate (MTX) (Bernal et. al., 1990). This dissertation research was done to identify the mechanism of MTX resistence correlated with SQMI protein levels and to understand the role of SQMI protein in MTX sensitivity. Primary cultures and uncloned cell lines of various lung carcinoma cell types show a correlation of MTX sensitivity with SQMI protein levels and MTX transpoert indicating a possible role of SQMI protein in MTX sentivity by increased MTX transport SQMI protein levels also correlated with the degree of differentiation of the lung cancer cell type. In bronchial epithelial cells induced toward squamous differentiation, SQMI protein was transiently induced during early stages of squamous differentiation of NBE cells concomitant with increased MTX sensitivity. MTX resistent cloned cell lines were developed in order to pin point the mechanism of resistance that correlates with SQMI protein levels. MTX resistant sublines developed from a clonally derived SqCHN cell line, SCC15S1, showed high correlation of SQMI protein expression with MTX transport but not with other mechanism of MTX resistence such as MTX-polyglutamylation and DHFR enzyme activity. Exposure of MTX resistent SCC15R1.3 to SQMI protein in the media led to increased MTX sensitivity and transport. Exposure of MTX resistant cells to liposomes containing SQM1 protein also increased MTX uptake and sensitivity. Transfection of SQM1 cDNA in a mammalian expression vector, p205, again increased MTX uptake and sensitivity of CEM lymphoblastic lymphoma cells and MTX resistant SCC1R1.3. |
521 ## - TARGET AUDIENCE NOTE | |
Target audience note | Department of Biochemistry |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | Library of Congress Classification |
Koha item type | Theses |
Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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Library of Congress Classification | Far Eastern University - Nicanor Reyes Medical Foundation | Far Eastern University - Nicanor Reyes Medical Foundation | Theses | 02/11/2015 | FMBIO19970001 | T000729 | 02/11/2015 | 07/22/2016 | Theses |