MARC details
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03467nam a22003257a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20240720152839.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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170111b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
MT 2010 0012 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Polly Chua-Chan, MD. ; Macario Reandelar MD. ; Mrs. Alicia A. Aldave, RMT, MHPEd |
| Relator term |
adviser |
| 111 ## - MAIN ENTRY--MEETING NAME |
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| Date of meeting |
March 2010 |
| Subordinate unit |
4MTB |
| 245 ## - TITLE STATEMENT |
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| Title |
The Effectiveness of kamias (averrhoa bilimbi) extract as decolorizer gram negative and gram positive bacteria in staining procedure / |
| Statement of responsibility, etc. |
Frederick Maigne M. Felizarte ; Trixia Marie B. Domingo ; Graciel S. Manalo ; Ezra Joanna B. Mandanas ; Ma. Cristina Sybyl F. Melendres ; Rushelle A. Obogne and Kimberly M. Silagan. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City |
| Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF, |
| Date of publication, distribution, etc. |
March 2010 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
27 pages: |
| Other physical details |
illustrations, tables, photos; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Abstract: This study is the effectiveness of the Averhoa Bilimbi (kamias) pure extract to be a decolorizer of Gram Positive and Gram Negative bacteria in Gram staining procedure. There are four basic steps in Gram staining which include applying a Primary stain-Crystal Violet, followed by adding a Mordant - Gram's Iodine, and then decolorize with 95% Ethyl Alcohol, and Counterstaining with Safranin - Basic Fuchsin. The Gram positive cell due to its multilayered nature of its peptidoglycan and has a thick cell wall should not be decolorized that will remain its purple color while the Gram negative cell lose its outer membrane because of its thin cell wall that will decolorized the Crystal Violet and will give a pink or red color after counterstain with Safranin. The study uses a bacterial suspension of E. coli as Gram bacteria and S. aureus as Gram positive bacteria. The Averhoa Bilimbi (kamias) extract is prepared in pure form. It is blended and sqeezed using cheesecloth and placed in a clean container. This study uses the grading system of 0, + and ++ to determine the degree of decolorization and integrity of the said bacteria. It is found out that E. coli can be decolorized in thirty, sixty and ninety seconds and its integrity remained intact in all fields seen with pink color. Statistically, it has no significant difference between the kamias and ethyl alcohol with a p-value of 1. However, in S. aureus it can be partially decolorized in thirty seconds and its integrity did not remained intact in all fields. Statistically, it means that there is a significant difference between kamias and ethyl alcohol with a p-value of 0.02. During sixty and ninety seconds, S. aureus cannot be decolorized and its integrity remained intact with violet color. Statistically, it means that there is no significant difference between the kamias and ethyl alcohol with a p-value of 1. It is concluded that the Averrhoa Bilimbi (kamias) pure extract can be used as a decolorizer and its integrity remained intact in all fields. |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - School of Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Felizarte, Frederick Maigne M. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Domingo, Trixia Marie B. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Manalo, Graciel S. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mandanas, Ezra Joanna B. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Melendres, Ma, Cristina Sybyl F. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Obogne, Rushelle A. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Silagan, Kimberly M. |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Theses |
