MARC details
| 000 -LEADER |
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| fixed length control field |
02765nam a22003497a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240720152840.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
170112b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
MT 2013 0007 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mr. Mark Ryan V. Domingo |
| Relator term |
adviser |
| 245 ## - TITLE STATEMENT |
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| Title |
The Effectiveness of Basella Rubra Stem as a Counterstain in Gram Staining / |
| Statement of responsibility, etc. |
Yasmin Marie Q. Astilla ; Maria Victoria Bianca R. Cala ; Mariell C. Ko ; Francis Dominic C. Lizardo ; Maria Cristina M. Mariano ; Maria Zennah P. Querol ; Camille C. Quinto ; Ana Mariella F. Rosales ; Kyla Nicole V. Valmonte and Gian Angelo A. Villalon. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City |
| Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF |
| Date of publication, distribution, etc. |
2013 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
33 pages: |
| Other physical details |
illustrations, tables, photos; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Abstract: Nowadays, most laboratories are using synthetic stains. Due to its many disadvantages, finding an alternative would benefit most of the laboratories as well as the personnel performing procedures involving staining. Based on research, plant-based dyes are said to be the least hazardous. Basellarubra, also known as "Alugbati", are known to contain anthocyyanin, which is an important component for staining. Anthocyanins are mainly found in the fruits and the flesh of a plant. In this research we extracted the anthocyanin and used it as a counterstain. Since the procedure was based on the routine Gram staining, we used the Safranin as the control. Gram-positive and Gram negative bacteria were used to be able to determine if the stain worked. The smears were observed and graded by its intensity. There were no organisms seen in any of the smears after staining. We concluded that the stain produced by the Alugbati can not be used as an alternative for counterstaining. There were certain factors that contributed to the said conclusion such as pH, buffer and mordant. Not all fruits that contain anthocyanin can be used as stain. The antibacterial and antifungal property of the fruit itself must also be considered and technical errors should be lessened |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - School of Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Astilla, Yasmin Marie Q. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cala, Maria Victoria Bianca R. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ko, Mariell C. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lizardo, Francis Dominic C. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mariano, Maria Cristina M. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Querol, Maria Zennah P. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Quinto, Camille C. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rosales, Ana Mariella F. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Valmonte, Kyla Nicole V. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Villalon, Gian Angelo A. |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Theses |
