MARC details
| 000 -LEADER |
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| fixed length control field |
02709nam a22002657a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
M BIO 2014 0003 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240720152846.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
170217b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Language of cataloging |
eng |
| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| Description conventions |
rda |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
english |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
M BIO 2014 0003 |
| 245 ## - TITLE STATEMENT |
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| Title |
HPLC analysis of the quercetin content of active hypouricemic hexane fractions of persea americana mill. leaves / |
| Statement of responsibility, etc. |
Section C; Joel Javate. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City: |
| Name of publisher, distributor, etc. |
Department of Biochemistry, FEU-NRMF, |
| Date of publication, distribution, etc. |
2014. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
39 pages: |
| Other physical details |
illustrations, tables, photos; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Abstract: Leaves of P. americana Mill. Contain significant amount of phenols and flavonoids which exhibit antioxidant and hypouricemic properties. This study investigated the presence of quercetin and other hypouricemic compounds in the active hexane fractions of P. americana.Pulverized P. americana leaves were soaked in 80% ethanol and evaporated via rotary evaporator. The crude ethanolic extract was partitioned between distilled water and hexane (1:6) using a separatory funnel. Two grams of hexane extract was subjected to vacuum liquid chromatography (VLC) for the separation of components based on their polarity. Thin layer chromatography (TLC) was employed to monitor the separation of components of the resulting fractions which yielded two active fractions. F1H (20%H:80%E) and F2H (60%H:40%E), as identified by the First Year Medical Students Section C1 2nd semester SY 2012-2013. High performance liquid chromatography (HPLC) analysis of the fraction showed significant peaks situated around the retention time recorded from the standard quercetin chromatogram, which demonstrates a positive result for the presence of quercetin in the sample. Percent (%) composition and concentrationof quercetin in F1H was 1.2655% and 0.006328mg/ml while 5.27598 % and 0.02638 mg/ml for F2H which clearly shows that F2H has greater % composition and quercetin concentration than F1H. Rf values 0.929,0.357, 0.610, 0.829 obtained from the fractions via TLC correspond to phytochemical compounds commonly found in P. american as in apigenin, luteolin, isorhamnetin, quercetin and rutin, respectively. Quercetin, a flavonol with known hypouricemic activity and flavonoids apigenin, rutin, luteolin, and isorhamnetin concurred to have possible contributions to the hypouricemic activity of P. americana |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - Department of Biochemistry & Nutrition |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Section C |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Javate, Joel, MD. |
| Relator term |
adviser |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Room Use |
