MARC details
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03564nam a22003617a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240720152909.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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170726b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
MT 2017 0006 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Alicia A. Aldave |
| Relator term |
adviser |
| 111 ## - MAIN ENTRY--MEETING NAME |
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| Date of meeting |
June 2017 |
| 245 ## - TITLE STATEMENT |
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| Title |
Chocolate Agar Plate Modification with Seed Extract from Cacao Tree (Theobroma Cacao L.) in Culturing Haemophilus Influenzae / |
| Statement of responsibility, etc. |
Wilniel Bryan F. Calibuso ; Ryan Kenneth G. Canoy ; Bernadette Jia M. Cruz ; Anne Beatrize U. Cua ; Bonn Joseph H. Dedace ; Ray Arden G. Demotica ; Anjunelle S. Esquivel ; Bryan E. Napuaga ; Chellie Mae D. Serrano and Maria Corina O. Velasco. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City |
| Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF |
| Date of publication, distribution, etc. |
June 2017 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
64 pages: |
| Other physical details |
illustrations, tables, photos; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Abstract: Isolation and identification of certain genus and species of bacteria can be done through growth in a culture medium. One example of such medium is Chocolate Agar Plate (CAP), a non-selective media wherein certain enrichment factors are added to promote or enhance growth of some fastidious bacteria. Haemophilus influenzae are small, non-motile gram negative coccobacilli, and are fastidious by nature. They require factors for growth such as Heme factor and Nicotine Adenine Dinucleotide factor. These requirements are provide in CAP to support its growth. Theobroma cacao is a small evergreen tree that is readily available in the Philippines. In many studies, it was proven that cacao has inhibitory effects to bacteria but not the genus Haemophilus. This study involves the cultivation of a purer culture of H. influenzae through the replacement of the inhibitory agent bacitracin with crude extract of cocoa to see if the modified chocolate agar plate is capable of producing results equal to the standard or greater in terms of suppressing the growth of other contaminant bacteria thus growth of purer culture of H. influenzae. Theobroma cacao seed were ground and extracted via ethanolic extraction the subjected to rotary evaporation that yielded the crude extract and incorporated to the CAP as the experimental plates, Bacitracin CAP served as the control. Three concentrations are prepared from the Theobroma cacao crude extract. 4 organisms were used. Haemophilus influenza, as the organism in focus in the study and other organisms which are the usual undesired organisms. The organisms were inoculated on both the plates and observed for the growth and number of colonies. Results showed that modified CAP with seed extract from cacao tree with the concentration of 3% and 5% have not shown selectivity to H.influenzae while 10% of cacao modified CAP have not shown its selectivity but was able to show how increasing concentration of cocoa extract affect the growth of the selected organism. |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - School of Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Calibuso, Wilniel Bryan F. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Canoy, Ryan Kenneth G. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cruz, Bernadette Jia M. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cua, Anne Beatrize U. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dedace, Bonn Joseph H. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Demotica, Ray Arden G. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Esquivel, Anjunelle S. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Napuaga, Bryan E. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Serrsno, Chellie Mae D. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Velasco, Maria Corina O. |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Theses |
