MARC details
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03532nam a22003737a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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PILC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20240720152913.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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170729b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
FEU-NRMF MEDICAL LIBRARY |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
MT 2017 0013 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mr. Normel Aldave |
| Relator term |
adviser |
| 111 ## - MAIN ENTRY--MEETING NAME |
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| Date of meeting |
June 2017 |
| 245 ## - TITLE STATEMENT |
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| Title |
Hylocereuscostaricencis (Dragon Fruit) Peel Extract as a Counterstain in Histological Staining / |
| Statement of responsibility, etc. |
Sheena Arrojo ; Lizette Anne Baratang :Alexis Pearl Despojo ; Jillan Libatique ; Clarisse Dale Gell Mallari ; Joi Esra Malundo ; Hatsumi Noda ; Justine Jaro Obligado ; Adrian Joseph Piasan ; Rhon Andrew Raneses and Isabella Chesca Tayag. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Fairview, Quezon City |
| Name of publisher, distributor, etc. |
School of Medical Technology, FEU-NRMF |
| Date of publication, distribution, etc. |
June 2017 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
30 pages: |
| Other physical details |
illustrations, tables, photos. |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacntent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes appendices and bibliographical references. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
ABSTRACT: Eosin, a synthetic dye that is used in routine hematoxylin and eosin (H&E) Staining technique in laboratories, has been reported to have effects on the human health and may potentially cause to the environment. This research aims to use natural products, specially the Dragon Fruit (Hylocereuspolyrhizus), to determine its efficacy as a counterstain. The dragon Fruit Extract will be obtained through a simple extraction and distillation method. Factors such as pH of the fruit, light, temperature and storage conditions will be taken into consideration as to preserve the color of the extract. The preparation of the tissue specimen, particularly the pork chop tissue undergone the routine procedure for tissue processing that uses paraffin and sectioning. The tissue staining procedure will follow the H&E Staining procedure for paraffin embedded sections. Twelve slides were used: nine of which were used to counterstain with Dragon Fruit Extract at different minutes; three other slides were used to counterstain with eosin. A Pathologist will evaluate the stained slides based on a criteria provided which includes: a) the degree of staining, b) the clarity of cellular components, and c) the intensity of the staining. The data collected was presented on a tabular form. Slides that were stained with Dragon fruit extract for 3 minutes and 5 minutes were graded as 3+ which is interpreted that the whole tissue was stained. Slides that were stained for 1 minute were graded 2+ which are interpreted as 50% of the tissue show staining color. In term clarity of the cellular components slides that were stained for 3 and 5 minutes were classified as clear thus, cellular components are visible. Slides that were stained for 1 minute were classified as not clear correlating the cellular components are not visible,. In terms of intensity, slides with 1 minute, 3 minute and 5 minutes were graded as contrasting meaning the stain differentiates cellular components from the rest to the tissue |
| 521 ## - TARGET AUDIENCE NOTE |
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| Target audience note |
Thesis - School of Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Arrojo, Sheena |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Baratang, Lizette Anne |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Despojo, Alexis Pearl |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Libatique, Jillan |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mallari, Clarisse Dale Gell |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Malundo, Joi Esra |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Noda, Hatsumi |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Obligado, Justine Jaro |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Piasan, Adrian Joseph |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Reneses, Rhon Andrew |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tayag, Isabella Chesca |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Theses |
