Crude methanol extract of Ipomoea Batatas L. Leaves as primary stain in gram stain technique / Jocel L. Antonio ; Camellie Grace A. Calisaan ; Caila Mae SD. Capuno ; Dylan Jason V. De Castro ; Angelica F. De Jesus ; Mary Rose S. Dela Cruz ; James Matthew M. Dimaano ; Thea Danille N. Drilon ; Beatriz D. Libunao ; Deanne Nicole Sangalang and Minerva Vrem D. Tominez.

Includes appendices and bibliographical references.

Abstract: Crystal Violet is more commonly known as a dye used in histological staining which is Gram's method of classifying bacteria. Despite of its known usefulness, it also acts as a mitolic poison and a potential carcinogen. While anthocyanin, being a useful colorant in many industries with its possible usefulness in being a colorant in the method of staining, has briught interest in the researchers being sweet potato leaves (Ipomoea batatas L.) as a subject of interest its possibility to be a primary stain in the Gram's stain method. This study determined whether an extract coming from sweet potato leaves is an effective primary stain, the method used in staining the sides is the Gram's stain method. It was done through a crude extract from the Department of Science and Technology-Chemical and Energy Division through methanol extraction, the extract was diluted in concentrations of 25%, 50%, 75%,and 100%. After which, the researchers inoculated isolated bacteria provided by the Far Eastern University - Nicanor Reyes Medical Foundation microbiology laboratory bonto slides which was used for the staining process. After the staining process of Staphylococcus aureus (gram positive ) and Escherichia coli (gram negative) in different concentrations of the extract as a primary stain. it showed that no color was able to adhere with the gram positive organism while no unusual results were given by the gram negative organism. This study concluded that anthocyanin from sweet potato leaves with its purpose as a primary stain was not able adhere to the organisms.

Thesis - School of Medical Technology