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Antimicrobial sensitivity of selected bacterial isolates to Prodigiosin / Erik Francis Rarugal ; Maryed Alday ; Isabelle Nicole Bagnes; Ma. Louren Deona ; Mikaelle Endrino ; James Jireh Grutas ; Reneeka Quilit, Janelyn Romarate ; Janna Katrina Silverio and Trisha Tan.

By: Contributor(s): Language: english Publication details: Fairview, Quezon City: School of Medical Technology, FEU-NRMF, 2019.Description: 42 pages: illustrations, tables, photos; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2019 0002
Summary: ABSTRACT: During the early times, clinical conditions caused by microorganisms were harmful and lethal. However, with the discovery of antibiotics, these diseases are now treated and prevented but the same bacteria are slowly developing a resistance which requires new antimicrobials to be introduced. In this study, the antimicrobial effectivity of the crude extract from the red pigment of Serratia marcescens was tested by using chloroform: methanol solvent system then evaporated using a water bath. The 0.5 grams of the crude extract was serially diluted with dimethyl sulfoxide (DMSO) then tested against standardized bacterial isolates. The turbidity was read with a spectrophotometer at 660 nm. Bacillus subtilis, Streptococcus pyogenes and Klebsiella pneumoniae have shown a general increase in turbidity. This implies that the bacteria continued to grow despite the addition of the crude extract. Therefore, the crude pigment extract is not an effective antimicrobial agent.
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Holdings
Item type Current library Call number Status Date due Barcode
Room Use Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2019 0002 c.1 (Browse shelf(Opens below)) Available T002037
Room Use Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2019 0002 c.2 (Browse shelf(Opens below)) Available T002038
Room Use Far Eastern University - Nicanor Reyes Medical Foundation Theses MT 2019 0002 c.3 (Browse shelf(Opens below)) Available T002039

Includes appendices and bibliographical references.

ABSTRACT: During the early times, clinical conditions caused by microorganisms were harmful and lethal. However, with the discovery of antibiotics, these diseases are now treated and prevented but the same bacteria are slowly developing a resistance which requires new antimicrobials to be introduced. In this study, the antimicrobial effectivity of the crude extract from the red pigment of Serratia marcescens was tested by using chloroform: methanol solvent system then evaporated using a water bath. The 0.5 grams of the crude extract was serially diluted with dimethyl sulfoxide (DMSO) then tested against standardized bacterial isolates. The turbidity was read with a spectrophotometer at 660 nm. Bacillus subtilis, Streptococcus pyogenes and Klebsiella pneumoniae have shown a general increase in turbidity. This implies that the bacteria continued to grow despite the addition of the crude extract. Therefore, the crude pigment extract is not an effective antimicrobial agent.

Thesis - School of Medical Technology

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