Ethanolic extract from Vitis Vinifera skin as contrast dye in detecting amoeba parasites / Sealtiel M. Bacolor ; Dixie Mae P. Bulac ; Princess Jasmine M. Carreon ; Benedict S. Dueñas ; Gail Nikole L. Ifurung, Patrick Lim ; Angela D. Manubay ; John Andrew M. Pineda ; Jericho Marlowe V. Ramiro ; Margarette U. Ruby and Hannah Eleanor Paz N. Servando.

Contributor(s):

Language: english Publication details: Fairview, Quezon City: School of Medical Technology, FEU-NRMF, 2019.Description: 79 pages: illustrations, tables, photos; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2019 0007

Summary: Abstract: In routine fecalysis, Lugol's Iodine is one of the most commonly used stains in laboratories. Tannins, a compaound that imparts a brown color on tissues and is usually extracted from grapes, has made its way in the field of staining technology. This study aims to determine if the tannin extract from grapes could be an effective contrast dye in staining the amoeba parasites namely: Entamoeba coli and Entamoeba histolytica. Another goal of the study is to determine which is better between the Lugol's Iodine and tannin extract. Tannin was extracted from the grapes' skin. The peeled grape skin was dried for 3 days, soaked in 95%ethanol, and was filtered. The filtrate was then extracted for tannins by Rotary Evaporation. The extract was then diluted with distilled water to produce four solutions of different concentration; 100%, 75%, 50%, 25%. Three Registered Medical Technologists read five slides each; four slides for the four dilutions, and one slide using Lugol's Iodine to establish the desired comparison. The slide is made by placing one drop of a solution, mixed with drop of preserved stool specimen. The results showed that for the 25% solution, three out of three medical technologists agreed that is poorly stained the amoeba parasites; for the 50% solution, three out of three medical technologists agreed that is perfectly stained the amoeba parasites; and for the 75% and 100% solution, two medical technologists agreed that it poorly stained the parasites, while one medical technologist agreed that both dilutions stained the parasites perfectly. This concludes that out of the four tannin solutions, the 50% tannin solution produced the best results. As for the comparison. three out of three Registered Medical Technologists agreed that Lugol's Iodine is still better than tannins as a contrast dye

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Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2019 0007 c.1 (Browse shelf(Opens below))	Available	T002058
Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2019 0007 c.2 (Browse shelf(Opens below))	Available	T002059
Room Use	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2019 0007 c.3 (Browse shelf(Opens below))	Available	T002060

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Previous	MT 2019 0006 c.3 Potential of Curcuma Longa (Turmeric) extract as an alternative to Eosin for periperal blood smear /	MT 2019 0007 c.1 Ethanolic extract from Vitis Vinifera skin as contrast dye in detecting amoeba parasites /	MT 2019 0007 c.2 Ethanolic extract from Vitis Vinifera skin as contrast dye in detecting amoeba parasites /	MT 2019 0007 c.3 Ethanolic extract from Vitis Vinifera skin as contrast dye in detecting amoeba parasites /	MT 2019 0008 c.1 Coconut oil and palm oil as substitute cleaning agents of Xylene in tissue processing /	MT 2019 0008 c.2 Coconut oil and palm oil as substitute cleaning agents of Xylene in tissue processing /	MT 2019 0009 c.1 Alugbati (Basella Rubra) Fruit Extract : A Possible Alternative to Hematoxylin for Cytologic Smears /	Next

Includes appendices and bibliographical references.

Abstract: In routine fecalysis, Lugol's Iodine is one of the most commonly used stains in laboratories. Tannins, a compaound that imparts a brown color on tissues and is usually extracted from grapes, has made its way in the field of staining technology. This study aims to determine if the tannin extract from grapes could be an effective contrast dye in staining the amoeba parasites namely: Entamoeba coli and Entamoeba histolytica. Another goal of the study is to determine which is better between the Lugol's Iodine and tannin extract. Tannin was extracted from the grapes' skin. The peeled grape skin was dried for 3 days, soaked in 95%ethanol, and was filtered. The filtrate was then extracted for tannins by Rotary Evaporation. The extract was then diluted with distilled water to produce four solutions of different concentration; 100%, 75%, 50%, 25%. Three Registered Medical Technologists read five slides each; four slides for the four dilutions, and one slide using Lugol's Iodine to establish the desired comparison. The slide is made by placing one drop of a solution, mixed with drop of preserved stool specimen. The results showed that for the 25% solution, three out of three medical technologists agreed that is poorly stained the amoeba parasites; for the 50% solution, three out of three medical technologists agreed that is perfectly stained the amoeba parasites; and for the 75% and 100% solution, two medical technologists agreed that it poorly stained the parasites, while one medical technologist agreed that both dilutions stained the parasites perfectly. This concludes that out of the four tannin solutions, the 50% tannin solution produced the best results. As for the comparison. three out of three Registered Medical Technologists agreed that Lugol's Iodine is still better than tannins as a contrast dye

Thesis - School of Medical Technology

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