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Coconut oil and palm oil as substitute cleaning agents of Xylene in tissue processing / Althea M. Bacayana ; Bettina Anne Louise I. Colobong ; Lyssa Mae E. De Belen ; Danzelle Macrine B. Collantes ; Marielle Coleen P. Domingo ; Jas Merain J. Fabello ; Glizel B. Maga ; Lou Modesselle W. Mandocdoc ; Joseph Angelo B. Racelis ; Marielle Erika C. Radin and Nathanielle Mae U. Rosal.

Contributor(s): Language: english Publication details: Fairview, Quezon City: School of Medical Technology, FEU-NRMF, 2019.Description: 55 pages: illustrations, tables, photos; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
LOC classification:
  • MT 2019 0008
Summary: Abstract: Xylene is the most commonly used clearing agent in tissue processing that can be toxic and carcinogenic especially in direct contact or long term exposure. The researchers aim to determine whether coconut oil and palm oil are effective substitutes for xylene as clearing agents in routine histhopathologic procedure. Twelve samples of pork tissue were divided into three experimental groups labelled A, B and C. The researchers used various organs of pig tissue specifically: lungs, large intestine, skeletal muscle and liver obtained from a local market. Group A was Cleared with xylene and group B and C with coconut oil and palm oil respectively. The tissue sections were then stained using hematoxylin and eosin (H&E stain). The results indicated that coconut oil and palm oil showed no significant difference with that of xylene thus exhibiting comparable clearing and staining capacity.
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Includes appendices and bibliographical references.

Abstract: Xylene is the most commonly used clearing agent in tissue processing that can be toxic and carcinogenic especially in direct contact or long term exposure. The researchers aim to determine whether coconut oil and palm oil are effective substitutes for xylene as clearing agents in routine histhopathologic procedure. Twelve samples of pork tissue were divided into three experimental groups labelled A, B and C. The researchers used various organs of pig tissue specifically: lungs, large intestine, skeletal muscle and liver obtained from a local market. Group A was Cleared with xylene and group B and C with coconut oil and palm oil respectively. The tissue sections were then stained using hematoxylin and eosin (H&E stain). The results indicated that coconut oil and palm oil showed no significant difference with that of xylene thus exhibiting comparable clearing and staining capacity.

Thesis - School of Medical Technology

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