Citrofortunella Microcarpa (Calamansi) Extract as White Blood Cell Diluting Fluid / Andrea Nicole Acebedo ; Vina Marie Advento ; Frances Beltran ; Maria Veronica Cortez ; Phoebe Laxamana ; Ronnie Miguel ; Erika Pacia ; Krizelbelle Sus ; Charisma Tolentino and Juan Angelo Verdillo.

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Publication details: Fairview, Quezon City School of Medical Technology, FEU-NRMF 2010Description: 19 page: illustration, tables; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

LOC classification:

MT 2010 0003

Summary: Abstract: The experimental study proposed Citrofortunella microcarpa (Calamansi) is to be used as an alternative White blood cell (WBC) diluting fluid for manual counts. Citrofortunella microcarpa (Calamansi) is preferably stored at refrigerator temperature and has a pH 2.4. The standard diluent in the clinical laboratory which is used to lyse red blood cells (RBC) is the dilute solutions of glacial acid. RBC`s are lysed in order to do manual white blood cell counts. Blood samples from 5 known individuals using either acetic acid (standard) and Citrofortunella microcarpa (Calamansi) extract was examined by three registered medical technologists and Red Blood Cells (RBC) lysis and the White Blood Cells (WBC) count were compared. The readings of two out of three Medical Technologists was used as the final result. The result of the study added a new information regarding the manual count for WBC which is calamansi extract as an alternative diluting fluid. Acetic acid showed 100% effective in the lysing of RBC while Calamansi extract showed to be ineffective, but RBC`s appeared as ghost cells wherein calamansi extract was not able to totally break the membrane of the RBC but had cause them to swell. Regarding in the preservation of the WBC, the result of P value was 0.37 which was not statistically significant. Therefore, Calamansi extract can preserve WBC and is comparable to Acetic acid.

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Item type	Current library	Call number	Status	Date due	Barcode
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2010 0003 c.2 (Browse shelf(Opens below))	Available		T001130
Theses	Far Eastern University - Nicanor Reyes Medical Foundation Theses	MT 2010 0003 (Browse shelf(Opens below))	Available		T000422

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Previous	MT 2010 0001 Anthocyanin Extracted from Eggplant Peel as Fluorescent Stain for Mycobacterium Tuberculosis /	MT 2010 0002 Brown Rice as an Enriched Substance for Culture Medium of Trichophyton Rubrum /	MT 2010 0002 c.2 Brown Rice as an Enriched Substance for Culture Medium of Trichophyton Rubrum /	MT 2010 0003 Citrofortunella Microcarpa (Calamansi) Extract as White Blood Cell Diluting Fluid /	MT 2010 0003 c.2 Citrofortunella Microcarpa (Calamansi) Extract as White Blood Cell Diluting Fluid /	MT 2010 0004 Degree of Utilization of Experimental Research Studies Done at Far Eastern University - Dr. Nicanor Reyes Medical Foundation, 1989-2009 /	MT 2010 0004 c.2 Degree of Utilization of Experimental Research Studies Done at Far Eastern University - Dr. Nicanor Reyes Medical Foundation, 1989-2009 /	Next

Includes appendices and bibliographical references.

Abstract: The experimental study proposed Citrofortunella microcarpa (Calamansi) is to be used as an alternative White blood cell (WBC) diluting fluid for manual counts. Citrofortunella microcarpa (Calamansi) is preferably stored at refrigerator temperature and has a pH 2.4. The standard diluent in the clinical laboratory which is used to lyse red blood cells (RBC) is the dilute solutions of glacial acid. RBC`s are lysed in order to do manual white blood cell counts. Blood samples from 5 known individuals using either acetic acid (standard) and Citrofortunella microcarpa (Calamansi) extract was examined by three registered medical technologists and Red Blood Cells (RBC) lysis and the White Blood Cells (WBC) count were compared. The readings of two out of three Medical Technologists was used as the final result. The result of the study added a new information regarding the manual count for WBC which is calamansi extract as an alternative diluting fluid. Acetic acid showed 100% effective in the lysing of RBC while Calamansi extract showed to be ineffective, but RBC`s appeared as ghost cells wherein calamansi extract was not able to totally break the membrane of the RBC but had cause them to swell. Regarding in the preservation of the WBC, the result of P value was 0.37 which was not statistically significant. Therefore, Calamansi extract can preserve WBC and is comparable to Acetic acid.

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